最新刊期
期 3 ， 2018
- 摘要：This study utilized antioxidant and basic single cell gel electrophoresis experiments to assess the effect of glycyrrhizin magnesium (Gly-Mg) on oxidative and DNA damage. ICR (Institute of Cancer Research) mice were divided into a control group (normal saline), irradiation control group (antioxidant experiment:normal saline+6.0 Gy; comet test:normal saline+7.2 Gy), and Gly-Mg group (antioxidant experiment:50 mg/kg+6.0 Gy, 75 mg/kg+6.0 Gy; comet test:50 mg/kg+7.2 Gy, 75 mg/kg+7.2 Gy). The activity of superoxide dismutase (SOD) and catalase (CAT), and the content of glutathione (GSH) and methylene dioxyamphetamine (MDA) in the serum, liver, and lung tissues of mice were detected using antioxidant kits. Results of the oxidative damage protection tests suggested that Gly-Mg can improve the activities of SOD and CAT, increase the content of GSH, and reduce the content of MDA in mouse serum and tissues. Meanwhile, results of the comet test showed that Gly-Mg groups had a significant protective effect on radiation-induced DNA damage in bone marrow compared with that in the irradiation control group (p < 0.01). In conclusion, Gly-Mg has protective effect against antioxidant and DNA damage.关键词：Glycyrrhizin magnesium (Gly-Mg);Radioprotection;Antioxidation;Comet assay
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发布时间：2021-12-10 - 摘要：The present study was to compare the X-ray sensitivity in human cervical cancer cell line HeLa, human hepatoma cell line HepG2, and human mucoepidermoid carcinoma cell line MEC-1. At 0.5, 4, and 24 h after X-ray irradiation with absorbed doses of 2, 4, 6, and 8 Gy, clonogenic assay, neutral comet electrophoresis, and immunofluorescence staining were used to evaluate the cell proliferation, DNA double-strand breaks (DSBs), and formation of phosphorylated histone H2AX (γH2AX) foci. The results showed that HeLa cells had the highest survival fraction (SF) for 2, 4, 6, and 8 Gy group at different time points after irradiation. Comet assay software project (CASP) analysis showed that DSBs increased with irradiation time, and absorbed dose. Tail moment (TM) increased most significantly at 4 h after irradiation, especially in the MEC-1 cells. The γH2AX phosphorylation level in the three cell lines reached 100% at 0.5 and 4 h after irradiation, and then gradually decreased by 24 h, this decline in γH2AX phosphorylation was the most obvious in HeLa cells. The above mentioned results showed that the combined use of clonogenic assay, neutral comet electrophoresis, and phosphorylated γH2AX foci detection can effectively predict the radiosensitivity of tumor cells.关键词：X-rays;Tumor cells;Radiosensitivity
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发布时间：2021-12-10 - 摘要：To study the molecular mechanism of the effect of a 0.4 mT power frequency magnetic field (PFMF) on the mobility of human amniotic epithelial (FL) cells, immunofluorescence labeling and western blotting were used to detect the distribution, expression, and activity of two key regulatory proteins, FAK and Paxillin, which function in the focal adhesion signaling pathway. Subsequently, cell migration was evaluated to verify whether FAK participated in PFMF-activated cell migration. The results showed that compared to the sham group, the number of FAK and Paxillin foci in the MF group increased at cell borders, which implied that the total number of focal adhesion spots had increased. Moreover, the total content of Paxillin increased in the PFMF-exposed group. Although the levels of FAK were not significantly changed, Y397 phosphorylation of FAK was significantly upregulated. When the activity of the epidermal growth factor receptor (EGFR) was inhibited by PD153035 (PD), or the phosphorylation of Y397-FAK was inhibited by FAK Inhibitor 14 (FI), the above-mentioned effects were no longer seen. Cell migration experiments showed that PFMF promoted cell migration through upregulating the FAK phosphorylation level, because the effect was sensitive to the inhibition of Y397-FAK phosphorylation. All evidence indicated that the effect of PFMF on cell mobility was similar to that of the EGFR ligand, epidermal growth factor (EGF). Therefore, it can be concluded that 0.4 mT PFMF regulates the formation and distribution of focal adhesions by activating EGFR, which subsequently activates FAK and Paxillin, resulting in an increase in cell mobility via lamellipodial extension.关键词：Power frequency magnetic field (PFMF);Focal adhesion;Cell migration;Epidermal growth factor receptor (EGFR);Focal adhesion kinase (FAK);Paxillin
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发布时间：2021-12-10
RADIOBIOLOGY AND RADIOMEDICINE
- 摘要：Six diaryliodonium salts with different structures were prepared. The conversion rate of epoxy resin (E-51) initiated by iodonium salt was determined via near-infrared spectroscopy. The effects of the substituents on the photocuring of E-51 induced by diaryliodonium salts, under a 395 nm LED light source, were investigated systematically. Molecular optimization of the six iodonium salts, as studied using the density functional theory, showed that the substituents had different effects on the LUMO (lowest unoccupied molecular orbital) and HOMO (highest occupied molecular orbital) energy levels of the diaryliodonium salts. Electrochemical experiments revealed that the substituents influenced the reduction potentials of the iodonium salts, and thus, affected the photoinduced charge transfer ability with the sensitizer. The free energy for charge transfer between the iodonium salts with different substituents and the isopropyl thioxanthones were calculated using the Rehm-Weller formula. The free energy changes were found to be -1.017, -0.720, -0.664, -0.577, -1.140, and -0.915 eV, and the possibility of charge transfer was discussed thermodynamically. In the case of the iodonium salts with different alkyl substituents, the smaller the steric hindrance of the alkyl group, the larger was the photoinitiation rate. The electron-withdrawing groups attached to the aromatic ring of the iodonium salts were found to further increase the photoinitiation rate.关键词：Epoxy resin;Diaryl iodonium salt;Photopolymerization;Charge transfer sensitization
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发布时间：2021-12-10 - 摘要：The ratio of[F-]/[Th(IV)] in the ThF4-LiCl-KCl molten salt system was adjusted by adding LiF and KF. The ratio of[F-]/[Th(IV)] reflected the concentration of F- in the molten salt. The effect of F- concentration on the electrolysis recycling rate of Th(IV) was examined. The recycling rate of Th(IV) decreased with increasing ratios of[F-]/[Th(IV)] from 4 to 12, indicating that increased F- concentration in the molten salt affected the precipitation of Th(IV) on the electrode. Chronopotentiometry was used to determine the diffusion coefficient of Th(IV) in the LiCl-KCl molten salt with different[F-]/[Th(IV)] ratios. The diffusion coefficient of Th(IV) initially decreased and subsequently increased, and a turning point was observed at a[F-]/[Th(IV)] ratio of 8. However, due to the increased F-concentration, the current density in the electrolysis process of Th(IV) was significantly reduced, resulting in a decreased recycling rate of Th(IV).关键词：ThF4;LiCl-KCl;Electrochemical recycling;[F-]/[Th (IV)]molar ratio
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发布时间：2021-12-10
RADIATION CHEMISTRY
- 摘要：This study examines the effects of low-dose ionizing radiation on peripheral hemocytes of the occupational population, and provides evidence for occupational protective measures. Electronic databases were searched for the relevant studies. A meta-analysis was performed using Stata12.0 software. The results indicated an increased risk of abnormal leukocyte and platelet count in radiation workers compared with that in nonradiation workers, with the combined odds ratio (OR) and 95% confidence interval (CI)=2.282 (1.845, 2.821) and 1.753 (1.348, 2.280), respectively, for the two groups. However, no statistically significant difference was observed in case of abnormal erythrocyte count. The subgroup analysis indicated an increase in the risk of abnormal leukocyte and platelet count (p < 0.05) with an increase in duration of service. In comparison, the increase in risk of abnormal erythrocyte count (p>0.05) was lower. Furthermore, the subgroup analysis, taking into account the type of work engaged in, revealed an increase in the abnormal leukocyte count for workers working with medical X-rays, interventional radiology, radiotherapy, nuclear medicine, and industrial inspection. Radiation workers involved in nuclear medicine were observed to be one of the most affected groups[OR=5.288, 95% CI (1.759, 15.892)], while those working with medical X-rays were relatively less affected[OR=1.988, 95% CI (1.178, 3.356)]. Our studies suggest that while low-dose ionizing radiation can increase the abnormal leukocyte and platelet count in radiation workers, it does not have a significant effect on the abnormal erythrocyte count.关键词：Ionizing radiation;Occupational exposure;Hemocyte;Meta analysis
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发布时间：2021-12-10
RADIATION DOSIMETRY
- 摘要：Ambroxol hydrochloride injections were irradiated with 60Co γ-rays at different absorbed doses (2, 4, 8, 16, 26, and 34 kGy), exposed to light (4 500±500) lx, and ultraviolet, and stored under high temperature (40, 60℃) for 10 d. The color, clarity, drug concentration, and related substances of injection were inspected. It was found that injection drug concentration significantly decreased under γ-irradiation, light exposure, UV radiation, and high temperature (60℃) (p < 0.01). The injection solution color changed to red brown due to the generation of unknown polarization-related substances under irradiation and UV environment, and drug content continued to decrease upon storage. It is shown that ambroxol hydrochloride injection is extremely unstable and is similarly affected under irradiation and UV environment.关键词：Ambroxol hydrochloride;Radiation;UV;High temperature;Light;High performance liquid chromatography (HPLC);Stability
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发布时间：2021-12-10 - 摘要：A computational fluid dynamics (CFD)-based three-dimensional offshore radionuclide-dispersion model was established to simulate the distribution of radionuclide concentration. Variations in the simulated concentration of tritium released from the La Hague nuclear fuel reprocessing plant demonstrated good agreement with surface-monitoring data, thereby effectively validating the proposed method. In addition, an accuracy comparison and analysis were performed using MARS (Hydrodynamical model for applications at regional scale). The results showed that the calculation results of this model agreed well with the monitoring data in a small scale (< 5 km). The error was controlled within 15%, and the simulation accuracy was slightly better than the MARS model. And results obtained reflected the ability of the proposed model to precisely simulate the radionuclide dispersion process performed in offshore waters with complex topography and hydrological conditions, thereby confirming its applicability in the prediction of the effects of radiation caused by nuclear accidents and/or conventional emissions. This would provide essential technical support during the decision-making process in the event of a nuclear emergency.关键词：Radionuclide-dispersion model;Computational fluid dynamics (CFD);Offshore areas;Nuclear emergency response
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发布时间：2021-12-10
RADIATION INTERDISCIPLINARY RESEARCH
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