Silk fibroin films irradiated by γ-rays with different doses (25, 50, 100, and 200 kGy) were implanted subcutaneously into SD rats. On the different dates (7th, 14th, 28th, 56th, and 84th day) after surgery, the rats were given euthanasia and the serum and tissue where the silk fibroin implanted were taken for further pathology tests and the concentration of IL-6 and TNF-α in the serum was also measured. The pathology results and the secretion of IL-6 & TNF-α showed that the silk fibroin did not induce significant immune reactions. The biodegradation rate increased more significantly with the increment of the absorbed doses and implantation time. In conclusion, γ-rays irradiation could increase the biodegradation rate of silk fibroin and almost could not change its biocompatibility.Cited