Bioinformatics analysis showed that pprM gene contained a Cold-shock DNA-binding domain. The pprM△CSD was obtained by Overlap-PCR technology. And then it was connected with the suicide vector pk18mobsacB. Before ligation, amplified fragment and the empty vector were digested by the restriction enzyme EcoR? and BamH?. The ligation products were transformed into E.coil JM109. Positive clones were selected and followed by restriction enzyme digestion and sequencing analysis results. The pprM gene DNA binding domain knockout vector recombinants were successfully constructed. The bioinformatics analysis shows that pprM gene DNA binding domain is associated with the radiation resistance function in Deinococcus radiodurans.Cited