After H1299 cells were treated with MG132, the cellular proliferation was detected by MTT assay; Transwell migration assay was used to determine the migration and invasion ability of H1299 cells in vitro.; changes of cell apoptosis were analyzed by flow cytometry assay; Western blot assay was employed to measure the protein expression. The results show that MG132 alone inhibits cell growth in H1299 cells, in a dose- and time-dependent manner; MG132 in combination with radiation significantly suppresses the migration and invasion of H1299 cells; MG132 enhances radiation-induced apoptosis in H1299 cells. The apoptosis rate of MG132+RT group is up to (11.44±0.90)%; the expression of MMP-2, -9, Bcl-2 is significantly suppressed by MG132 in combination with radia-tion, while the expression of Bax is up-regulated relatively. The present findings demonstrate that MG132 inhibits the cell growth in lung cancer, the migration and invasion ability of H1299 cells treated by MG132 in combination with radiation is significantly suppressed, while the apoptosis rate increases relatively. The mechanism may be associated with MMPs and Bcl-2 down-regulation, as well as Bax up-regulation.Cited