To investigate the effects of AS1411 on cell proliferation-toxic and radiosensitivity on cervical cancer HeLa cells, CCK-8 assay was used to determine cell proliferation-toxic effects on HeLa cells, and the effect of AS1411 on the radiosensitivity of the cells was observed by clonogenic assay. The results showed that the cell toxicity of AS1411 was very small, and all the survival rates of HeLa cells were more than 95% under the condition of  different concentration of AS1411 (0 nmol/L, 50 nmol/L, 100 nmol/L, 250 nmol/L and 500 nmol/L) and the cells for 24 h, 48 h and 72 h, respectively. Clonogenic assay result shows that AS1411 combined with X ray irradiation can decrease the clonogenic survival rate of HeLa cells, and the sensitive enhancement ratio (SER) of HeLa cells are 1.06, 1.58 and 1.89, respectively with different drug concentrations of 100 nmol/L, 250 nmol/L and 500 nmol/L. This study proves AS1411 might enhance the radiosensitivity of the HeLa cells.Cited