The ,Saccharomyces Cerevisiae, YE0 was mutated using high-energy-pulse-electron (HEPE) beam. After ethanol stress and determination of the alcohol yield by gas chromatographs, the mutant YF1 with high alcoholic yield was obtained. The results showed that under the optimized fermentation conditions (34℃ as the fermentation temperature, 72 h as the fermentation time and 30% as the glucose concentration), the alcoholic yield of YF1 was 15.57% which was 58.23% higher than that of the original strain YE0(9.84%) under the same conditions. The growth rate and lethal temperature of the mutant YF1 were obviously enhanced to the original strain YE0. The mutant YF1 has a great potential application in industrial production of alcohol. And it can also be used as the original strain for further mutagenesis to get the strain of higher alcoholic yield.