Li Hongyu, Li Chenghua, Ding Xinchun, et al. A function of mutagensis on rhodotorula ry strain irradiated by heavy ion[J]. Journal of Radiation Research and Radiation Processing, 2004,22(1):56-60.
Li Hongyu, Li Chenghua, Ding Xinchun, et al. A function of mutagensis on rhodotorula ry strain irradiated by heavy ion[J]. Journal of Radiation Research and Radiation Processing, 2004,22(1):56-60.DOI:
应用重离子加速器的50Mev/u,12,C,6+,重离子对胡萝卜素生产菌-红酵母(,Rhodotorula, RY Strain )进行辐照处理,经酵母发酵实验,发现50Mev/u,12,C,6+,重离子对胡萝卜素生产菌-红酵母具有诱变作用。初步筛选到了胡萝卜素产量有变化的辐照变异菌株,并对这些辐照变异菌株进行了RFLP(限制性片段长度多态性)和RAPD(随机扩增DNA多态性)分析,这些工作为工业上利用重离子对胡萝卜素生产菌进行诱变育种展现了新的前景。
Abstract
In this paper, red yeast (,Rhodotorula, RY Strain) that produces carotene is irradiated by 50Mev/u,12,C,6+, heavy ion from Heavy Ion Accelerator in IMP. Fermentation tests show that 50Mev/u,12,C,6+, heavy ion has a mutagenesis effect on the red yeast. some strains of red yeast with changed production of carotene were found by screening. Meanwhile, by RFLP and RAPD analysis, we have a further evidence that heavy ion can cause mutagenesis in ,Rhodotorula, RY Strain. This presents a new prospect for the mutagenesis breeding by heavy ion in industry.
关键词
重离子红酵母诱变作用RFLPRAPD
Keywords
heavy ionRhodotorula RY StrainMutagenesis effectRFLPRAPD