γ射线和紫外线照射对白血病K<sub>562</sub>细胞株中ppGalNAcT2 mRNA表达的影响

范雁; 吴士良; 徐岚; 陈克平; 仇灏

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γ射线和紫外线照射对白血病K₅₆₂细胞株中ppGalNAcT2 mRNA表达的影响

研究论文 | 更新时间：2023-03-30

- γ射线和紫外线照射对白血病K₅₆₂细胞株中ppGalNAcT2 mRNA表达的影响
- Effects of γ-rays and ultraviolet on the expressions of ppGalNAcT2 in K₅₆₂ leukaemia cell lines
- 辐射研究与辐射工艺学报 2004年22卷第6期页码：361-364
- 作者机构：
  
  1.苏州大学医学院生物化学与分子生物学教研室　苏州　215007
- 作者简介：
  
  范雁，女，1968年5月出生，2003年在苏州大学医学院获硕士学位，肿瘤分子生物学专业，讲师
  吴士良
- 基金信息：
  
  国防科工委国防基础科研基金(2003044);江苏省高校自然科学基金(01KJB310001);苏州大学医学发展基金(2003)
- DOI：
  中图分类号：
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范雁, 吴士良, 徐岚, 等. γ射线和紫外线照射对白血病K₅₆₂细胞株中ppGalNAcT2 mRNA表达的影响[J]. 辐射研究与辐射工艺学报, 2004,22(6):361-364.

FAN Yan, WU Shiliang, XU Lan, et al. Effects of γ-rays and ultraviolet on the expressions of ppGalNAcT2 in K₅₆₂ leukaemia cell lines[J]. Journal of Radiation Research and Radiation Processing, 2004,22(6):361-364.
范雁, 吴士良, 徐岚, 等. γ射线和紫外线照射对白血病K₅₆₂细胞株中ppGalNAcT2 mRNA表达的影响[J]. 辐射研究与辐射工艺学报, 2004,22(6):361-364. DOI：

FAN Yan, WU Shiliang, XU Lan, et al. Effects of γ-rays and ultraviolet on the expressions of ppGalNAcT2 in K₅₆₂ leukaemia cell lines[J]. Journal of Radiation Research and Radiation Processing, 2004,22(6):361-364. DOI：

摘要

观察了γ射线、紫外线对白血病细胞株K,562,中多肽：N−乙酰氨基半乳糖转移酶2（ppGalNAcT2，E.C2.4.1.41）基因mRNA表达的影响，初步探讨ppGalNAcT2与肿瘤发生、发展的相关性。以不同剂量的,60,Coγ射线、紫外线分别照射K,562,细胞，继续培养12h后将细胞收集，采用逆转录PCR （RT−PCR）法检测ppGalNAcT2 mRNA的表达差异变化。结果显示，γ射线、紫外线照射均可抑制白血病细胞ppGalNAcT2在mRNA水平的表达，提示此两种辐照可能与肿瘤细胞受照射后的浸润和转移性能有关，ppGalNAcT2与此性状间有一定的相关性。

Abstract

To observe the effect of γ-irradiation and UV on the mRNA expressions of ppGalNAcT2 (polypeptide N-acetylgalactosaminyltransferase-2) in K,562, leukaemia cells and study the relation of ppGalNAcT2 to tumor’s generating and developing. K,562, cells were exposed to different doses of γ-rays and ultraviolet, the expressions at mRNA level of ppGalNAcT2 were examined by reverse transcription polymerase chain reaction (RT-PCR) within 12h. The results showed that the mRNA expressions of ppGalNAcT2 decreased evidently in two irradiated groups, and they were negatively relative to the dosage. The mRNA expressions of ppGalNAcT2 in K,562, leukaemia cells could be inhibited by radiation , it is postulated that ppGalNAcT2 might relate to some biological specificity of cancer cells, such as tumor’s invasion and migration.

关键词

γ射线紫外线白血病K562细胞株多肽：N−乙酰氨基半乳糖转移酶2

Keywords

γ-irradiationultravioletK562 leukaemia cellsppGalNAcT2

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γ射线和紫外线照射对白血病K562细胞株中ppGalNAcT2 mRNA表达的影响

Effects of γ-rays and ultraviolet on the expressions of ppGalNAcT2 in K562 leukaemia cell lines

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摘要

Abstract

关键词

Keywords

references

γ射线和紫外线照射对白血病K₅₆₂细胞株中ppGalNAcT2 mRNA表达的影响

Effects of γ-rays and ultraviolet on the expressions of ppGalNAcT2 in K₅₆₂ leukaemia cell lines