用HPRT基因突变及CBMN研究AT细胞高辐射敏感性

罗加林; 曹建平; 朱巍; 刘芬菊; 王明明; 周献锋; 朱财英; 郑斯英

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用HPRT基因突变及CBMN研究AT细胞高辐射敏感性

研究论文 | 更新时间：2023-03-30

- 用HPRT基因突变及CBMN研究AT细胞高辐射敏感性
- Study on high radiosensitivity of AT cells by HPRT gene mutation and cytokinesis-block micronucleus method
- 辐射研究与辐射工艺学报 2004年22卷第6期页码：365-369
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院放射生物学教研室　苏州　215007
- 作者简介：
  
  罗加林，男，1978年11月出生，苏州大学在读硕士，从事辐射遗传学研究
  曹建平
- 基金信息：
  
  国家自然科学基金(30170288);江苏省2004年度研究生创新计划项目(Xm04-67);苏州大学医学发展基金重点项目(EE126032)
- DOI：
  中图分类号：
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罗加林, 曹建平, 朱巍, 等. 用HPRT基因突变及CBMN研究AT细胞高辐射敏感性[J]. 辐射研究与辐射工艺学报, 2004,22(6):365-369.

LUO Jialin, CAO Jianping, ZHU wei, et al. Study on high radiosensitivity of AT cells by HPRT gene mutation and cytokinesis-block micronucleus method[J]. Journal of Radiation Research and Radiation Processing, 2004,22(6):365-369.
罗加林, 曹建平, 朱巍, 等. 用HPRT基因突变及CBMN研究AT细胞高辐射敏感性[J]. 辐射研究与辐射工艺学报, 2004,22(6):365-369. DOI：

LUO Jialin, CAO Jianping, ZHU wei, et al. Study on high radiosensitivity of AT cells by HPRT gene mutation and cytokinesis-block micronucleus method[J]. Journal of Radiation Research and Radiation Processing, 2004,22(6):365-369. DOI：

摘要

研究了毛细血管扩张性共济失调症（ataxia−telangiectasia, AT）患者皮肤的成纤维细胞系AT5BIVA（AT细胞）的高辐射敏感性。以源于正常人皮肤的成纤维细胞系GM0639（GM细胞）为对照，利用次黄嘌呤磷酸核糖转移酶基因（Hypoxanthine phospho−ribosyl transferase, hprt）位点突变分析技术及胞质分裂阻滞微核法（Cytokinesis−Block micronucleus method，CBMN），在AT细胞和GM细胞经,60,Co γ射线0、1、2、3、4Gy照射后，观察比较AT细胞和GM细胞之间hprt基因位点突变频率（hprtMF）、微核率（MNF）及微核细胞率（MNCF）的差异，并分别进行曲线拟合。在各剂量点，AT细胞hprt基因突变频率、微核率及微核细胞率均明显高于GM细胞，其差别具有显著性统计意义（,p,<,0.01）；AT和GM细胞hprt基因突变频率、微核率及微核细胞率均与照射剂量呈正相关，可拟合成剂量效应直线方程,y,=a+bx。结果表明，毛细血管扩张性共济失调症患者AT细胞辐射敏感性显著高于GM细胞，具有高辐射敏感性。

Abstract

The high radiosensitivity of fibroblast cells (AT5BIVA cells) from the skin of the Ataxia- telangiectasia (A-T) patients were studied. Using Hypoxanthine phospho-ribosyl transferase gene mutation analysis and Cytokinesis-Block micronucleus method, the hprt mutation frequence(hprtMF), the micronucleus frequencies (MNF)and micronucleus cell frequencies(MNCF)of AT and GM cells irradiated with 0, 1, 2, 3 and 4Gy of ,60,Coγ-rays were observed. The difference of hprtMF, MNF and MNCF between AT and GM cells were analyzed, and dose-response curves of hprtMF, MNF and MNCF in AT and GM cells were also established. After exposure to 0, 1, 2, 3 and 4Gy of ,60,Coγ-rays, hprtMF, MNF and MNCF were significantly higher in AT cells than those in GM cells(,p,<,0.01). In the two cell types, hprtMF MNF and MNCF had a positive correlation with dose, and their linear regression equations were ,y,=a+bx. These results show that the radiosensitivity of AT cells is significantly higher than that of GM cells and AT cells are extremely sensitive to radiation.

关键词

毛细血管扩张性共济失调症高辐射敏感性次黄嘌呤磷酸核糖转移酶基因胞质分裂阻滞微核法

Keywords

Ataxia-telangiectasiaHigh radiosensitivityHypoxanthine phospho-ribosyl transferaseCytokinesis- Block micronucleus method

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