ZHANG Yiqing, LU Zhaoxin, ZOU Xiaokui. 15 keV N+ implantation to induce mutation of protopectinase-producing Aspergillus sp.[J]. Journal of Radiation Research and Radiation Processing, 2005,23(3):140-144.
ZHANG Yiqing, LU Zhaoxin, ZOU Xiaokui. 15 keV N+ implantation to induce mutation of protopectinase-producing Aspergillus sp.[J]. Journal of Radiation Research and Radiation Processing, 2005,23(3):140-144.DOI:
Protopectinase will be of potential use in industry. The strain of protopectinase-producing, Aspergillus sp., XZ-131 was mutagenized by 15keV N,+, ion implantation, with a dose range of 0.52×10,15, ion/cm,2, to 4.68×10,15, ion/cm,2,. From the ion implanted ,Aspergillus sp., XZ-131, the Strain Z-25, which yields high activity protopectinase, was screened out. The protopectinase activity increased by 179% and the protopectinase (U/g dry cell) increased by 84%. In addition, the exponential phase of, Aspergillus sp., Z-25 was 24 hours shorter than the Strain XZ-131. The ,Aspergillus sp., Z-25 yielded high protopectinase activity during stationary phase, the highest protopectinase activity was achieved after 72 h fermentation.
关键词
离子注入原果胶酶诱变筛选育种发酵
Keywords
Ion implantationProtopectinaseMutationScreeningBreedingFermentation process