1.湘潭大学食品与生物工程系 湘潭 411105
2.武汉大学生命科学院 武汉 430072
冯波,男,1976年8月出生,1998年毕业于武汉大学,生物化学专业,讲师,在读硕士研究生
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冯波, 曾虹燕, 唐裕芳, 等. 60Co辐照聚苯乙烯微孔板在抗−HCV ELISA中的应用[J]. 辐射研究与辐射工艺学报, 2005,23(6):321-324.
FENG Bo, ZENG Hongyan, TANG Yufang, et al. Application of 60Co γ−ray irradiated polystyrene microplate for anti-HCV ELISA[J]. Journal of Radiation Research and Radiation Processing, 2005,23(6):321-324.
为了探讨,60,Co γ射线辐照对聚苯乙烯微孔板吸附小分子多肽的影响,建立了检测抗−丙型肝炎病毒(Hepatitis C virus,HCV)的间接酶联免疫法(Enzyme−linked immunosorbent assay,ELISA)。分别用未经辐照处理、紫外线(Ultraviolet ray,UV)辐照处理和,60,Co辐照处理的微孔板吸附重组HCV抗原,再配以辣根过氧化物酶标记的羊抗人IgG,考察其灵敏度、特异性、均一性和稳定性等相关指标。实验结果表明,经8kGy,60,Co辐照处理的聚苯乙烯微孔板可改善酶联免疫法的测定效果,检测灵敏度和均一性均有所提高。
In order to explore the effect of ,60,Coγ−ray irradiation on minor polypeptides absorption of polystyrene microplate, an indirect ELISA detection of anti−HCV was established,60,Co γ−ray irradiated polystyrene microplates and the controls (without irradiation or UV−irradiated) were applied to absorb recombinant HCV antigens respectively. Cooperated with Bovine antihuman IgG labed HRP, their related indices of sensitivity, specificity, homogeneity and stability were determinated. The results indicated that, optimum dose of the γ−ray irradiation is 8kGy, and compared with the controls, detection sensitivity and homogeneity of the polystyrene microplate irradiated to 8kGy could be improved markedly.
酶联免疫法60Co γ射线小分子多肽重组丙型肝炎病毒抗原
ELISA60Coγ−rayMinor polypeptidesRecombinant HCV antigens
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