ZHANG Ning, YU Long, YANG Lifeng. Screening on the higher yield of transaminase producing Escherichia coli with the metal ion implantation[J]. Journal of Radiation Research and Radiation Processing, 2006,24(2):125-128.
ZHANG Ning, YU Long, YANG Lifeng. Screening on the higher yield of transaminase producing Escherichia coli with the metal ion implantation[J]. Journal of Radiation Research and Radiation Processing, 2006,24(2):125-128.DOI:
Mutagenic effects of the transaminase producing bacterium ,Escherichia coli, U12-3-19 by low energy metal ion (Ti,2+, ) implantation were investigated. And the different mutagenic effects of ,E.coli, irradiated with UV and low-energy ions were compared. The results showed that the ,E.coli, after the ion implantation could achieve higher rate of mutation and wide mutational spectrum. A high yield L-phe strain has been bred from this. In the shaking flask, the L-phe productivity of strain U12-25-116-J75 increased by 22.67% than of the comparison strain with the PPA concentration of 20g/L.
关键词
金属离子注入诱变选育L-苯丙氨酸
Keywords
Metal ion implantationMutationBreedL-phenylalanine