LIU Qingjie, CHEN Deqing. Rolling circle amplification and its application in biomedicine[J]. Journal of Radiation Research and Radiation Processing, 2007,25(1):5-9.
LIU Qingjie, CHEN Deqing. Rolling circle amplification and its application in biomedicine[J]. Journal of Radiation Research and Radiation Processing, 2007,25(1):5-9.DOI:
Rolling circle amplification (RCA) is an isothermal amplification method. The DNA can be amplified exponentially in a short time period. It can be used in detection of rare molecular. RCA is used to amplify not only the circular DNA or RNA, but also linear DNA and even whole genome DNA. At present, RCA is mainly used in fields of whole genome amplification, sequencing, single nucleotide polymorphism, DNA microarray, and protein microarray analysis etc.
关键词
滚环扩增全基因组分析单核苷酸多态性芯片
Keywords
Rolling circle amplificationWhole genome analysisSingle nucleotide polymorphismMicroarray