1.中国科学院等离子体物理研究所 合肥 230031
杨倩,女,1981年12月出生,2004年毕业于西北大学生命科学学院,现为中国科学院等离子体物理研究所在读硕士研究生
郑之明
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杨倩, 许永建, 郑之明. 空气负离子对阿维链霉菌发酵周期及产量的影响[J]. 辐射研究与辐射工艺学报, 2007,25(1):10-14.
YANG Qian, XU Yongjian, ZHENG Zhiming. The influence of air ions on the fermentation cycle and antibiotic output of streptomyces avermitilis[J]. Journal of Radiation Research and Radiation Processing, 2007,25(1):10-14.
为了研究低浓度空气离子对微生物的效应,利用低浓度空气离子对固体培养基上,Streptomyces avermitilis,作用,分析了其次生代谢产物产生时间早晚,各组分含量变化,并且初步研究有效成分含量提高的机理。用浓度为5×10,3,/cm,3,的空气负离子连续十天处理固体培养基上的阿维链霉菌,经过高效液相色谱(High performance liquid chromatography,HPLC)检测后发现:经过处理,该菌株提早四天到达阿维菌素(Avermectins, AVM)产素平台期,有效成分B1a和B1b提高约20%,而无效成分含量无显著提高。经过分析,低浓度空气负离子可有效缩短该菌种的发酵周期,提高有效成分含量,刺激次生代谢过程。
Effect of low concentration negative air ions on ,Streptomyces avermitilis, on solid culture medium was studied. The microorganism was treated consecutively with negative air ions of 5×10,3,/cm,3, in 10 days. It was found that under the treatment,Streptomyces avermitilis, reached the plat-stage production 4 days earlier than the control. Moreover, quantities of both effective contents of B1a and B1b increased by about 20%, while no remarkable changes in the ineffective contents were observed. Repeated experiments prove that the negative ion treatment is a useful way to shorten long fermentation cycle and to increase antibiotic production.
负离子阿维链霉菌阿维菌素
Negative ionsStreptomyces avermitilisAvermectin
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