YANG Yingge, LI Wen, LIU Dan, et al. Breeding L(+)-lactic acid high productive mutant from xylose by nitrogen ions[J]. Journal of Radiation Research and Radiation Processing, 2007,25(2):80-84.
YANG Yingge, LI Wen, LIU Dan, et al. Breeding L(+)-lactic acid high productive mutant from xylose by nitrogen ions[J]. Journal of Radiation Research and Radiation Processing, 2007,25(2):80-84.DOI:
In order to obtain higher L(+)-lactic acid yield strain fermentating from xylose, the original strain ,Rhizopus oryzae, RLC41-6 was mutated by 10keV N,+, ion implantation. A mutant strain RQ4012 was obtained. After 72h shake-flask cultivation, the concentration of L(+)-lactic acid reached 74.37g/L, and the productivity was 1.03g/(L.h). Its lactic acid yield was 160% higher than that of the original one, and the mutant strain has high genetic stability.