低剂量X射线对小鼠睾丸生精细胞中凋亡诱导因子变化的影响

王志成; 李艳博; 龚平生; 刘淑春; 刘扬; 康顺爱; 赵刚; 龚守良

您当前的位置：

首页 >

文章列表页 >

低剂量X射线对小鼠睾丸生精细胞中凋亡诱导因子变化的影响

研究论文 | 更新时间：2023-03-30

- 低剂量X射线对小鼠睾丸生精细胞中凋亡诱导因子变化的影响
- The effect on the change of apoptosis inducing factor in mouse testis spermatogeniccells irradiated with low dose X-rays
- 辐射研究与辐射工艺学报 2008年26卷第1期页码：28-32
- 作者机构：
  
  1.吉林大学公共卫生学院卫生部放射生物学重点实验室　长春　130021
  2.吉林大学分子酶学工程重点实验室　长春　130023
- 作者简介：
  
  王志成，男，1976年8月出生，2005年于吉林大学获硕士学位，讲师，放射医学专业
  龚守良
- 基金信息：
- DOI：
  中图分类号：
扫描看全文
王志成, 李艳博, 龚平生, 等. 低剂量X射线对小鼠睾丸生精细胞中凋亡诱导因子变化的影响[J]. 辐射研究与辐射工艺学报, 2008,26(1):28-32.

WANG Zhicheng, LI Yanbo, GONG Pingsheng, et al. The effect on the change of apoptosis inducing factor in mouse testis spermatogeniccells irradiated with low dose X-rays[J]. Journal of Radiation Research and Radiation Processing, 2008,26(1):28-32.
王志成, 李艳博, 龚平生, 等. 低剂量X射线对小鼠睾丸生精细胞中凋亡诱导因子变化的影响[J]. 辐射研究与辐射工艺学报, 2008,26(1):28-32. DOI：

WANG Zhicheng, LI Yanbo, GONG Pingsheng, et al. The effect on the change of apoptosis inducing factor in mouse testis spermatogeniccells irradiated with low dose X-rays[J]. Journal of Radiation Research and Radiation Processing, 2008,26(1):28-32. DOI：

摘要

采用链霉亲和素-生物素-过氧化物酶复合物法（Strept actividin-biotin complex，SABC），观察低剂量X射线照射后小鼠睾丸各类生精细胞中（Apoptosis inducing factors，AIF）的表达变化；采用逆转录多聚酶链反应（Reverse transfer polymerase chain reaction，RT-PCR）法观察AIF的mRNA水平变化。研究低剂量X射线对小鼠睾丸生精细胞中凋亡诱导因子AIF蛋白及mRNA水平的影响。研究发现，0～0.2Gy照射后，小鼠睾丸各类生精细胞不同程度表达AIF，以精原细胞和精母细胞表达为主，精子细胞和精子则表达相对较少。AIF表达量与吸收剂量有相关性，0.075Gy照射组表达量最大，且发现AIF蛋白的表达随时间推移而增强，12h达到峰值，然后下降，但仍高于0h组。RT-PCR结果显示，低剂量照射12h后，0.1Gy剂量组AIF mRNA表达量最大。而0.075Gy照射后0～24h，其mRNA表达量逐渐增大，在24h到达峰值。所以，低剂量X线照射诱导小鼠睾丸生精细胞中AIF蛋白及mRNA表达与吸收剂量和表达时间有一定的相关性。

Abstract

In order to investigate the effects of low dose irradiation on the expression of apoptosis inducing factors (AIF) protein and its mRNA in spermatogenic cells of mouse testis, immunohisto-chemical technique (SABC) and RT-PCR was used to observe the change of AIF protein and its mRNA expressions in the spermatogenic cells of the mouse testis irradiated with X-rays at different absorbed doses. After irradiation with 0~0.2Gy, the AIF protein expression occurred principally in spermatogonia and spermatocytes and less in spermatids and spermazoa. It was found that the AIF protein expressions increased with irradiation doses and reached the highest at absorbed dose of 0.075Gy, and then decreased gradually along with increasing doses. It was also found that the expression increased with the expression time and reached its top at 12h after irradiation, and then it decreased gradually but was higher than that of at 0h. In the RT-PCR, it was observed that the largest mRNA expressions occurred at 0.1Gy while irradiation doses changed from 0 to 0.2Gy and the peak of AIF mRNA expressions was at 24h after irradiation with 0.075Gy. As a conclusion, the expressions of AIF protein and its mRNA in spermatogenic cells of mouse testis induced by low-dose irradiation have some relationship with absorbed doses and expression time after irradiation with X-rays.

关键词

凋亡诱导因子mRNA电离辐射睾丸生精细胞

Keywords

Apoptosis inducing factors (AIF)mRNAIrradiationTestisSpermatogenic cell

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

毫米波辐射对雄性小鼠生殖系统的影响

电磁脉冲辐射对小鼠睾丸Occludin表达的影响

电磁脉冲辐射对小鼠睾丸TGF-βs表达的影响

PC/Irradiated HUPEA共混物力学性能研究

电磁脉冲辐射对小鼠睾丸Smads表达的影响