To detect the rate of lymphocyte Chromosome aberration by conventional chromosome production and ultraviolet plus Giemsa technology (UpG), after the human peripheral blood irradiated by ,60,Co γ-ray, and the dose-response relationship of the rate of chromosome aberration was discussed. The feasibility of UpG applied in dose estimation was discussed. The results show that the rate of chromosome aberration is closely related to dose (,P,<,0.05).Compared the conventional method to UpG, the result of UpG is higher, and a extremely statistically difference was observed between two methods (,P,<, 0.05). These results show that a well relationship of dose-response can be established by UpG, and accurate and sensitive.