In order to study the peroxidation reaction of erythrocyte and erythrocyte membrane and their protection with Vc involved in radiation from UV, alternate biological model has been used with erythrocyte and erythrocyte membrane, which is isolated from fresh pig blood. Hemolysis in erythrocyte and peroxidation in erythrocyte and erythrocyte membrane has been measured by the spectral photometer and the thiobarbituric acid (TBA) reaction. With the increment of UV radiation dose and deposited time, the hemolysis in erythrocyte increases, and the peroxidation of erythrocyte membrane also increases when radiation dose is up to some value. However, the peroxidation of erythrocyte hasn’t been observed while radiation dose is low. In our study, the bigger concentration of Vc, the more obvious protection to erythrocyte membrane. But the protection of Vc to the erythrocyte is not evidence.