In this paper the mutation of lycopene producing strain,B.trispora,(−) by use of low energy N,+, ion implantation,was studied. Experimental results show that higher mutation rate and wider mutation spectrum have been obtained after ,B.trispora, (−) being implanted and four lycopene high yielding strains ,B.trispora, (−) BH3-701 ,et al, have been screened out. It has been found that the average production of lycopene increases by 50% compared with that of original strain after five passages in shaking flasks. The highest yield strain BH3-701 can accumulate lycopene in the later stage and increase production efficiency greatly.