In order to investigate the protective effects of glucosinolate on the mice induced by ,12,C,6+, ion beams the mice were fed orally with crude extracts of glucosinolate in various doses for two weeks, and then were whole-body irradiated with 2Gy ,12,C,6+, ions. At 8h after irradiation the cell cycle status of the cells obtained from mice brain, lung and liver were measured by flow cytometry. The frequencies of cells with tail moment and the tail lengths were determined by the single cell gel electrophoresis. Compared with irradiation only group the percentages of cells in G,0,/G,1, phase decreased statistically significantly, whereas the percentages in G,2,/M and S phases increased (,p,<,0.05) in groups irradiated and treated with glucosinolate in lower doses. Similarly, the frequencies of cells with tail moment and the tail lengths induced by ,12,C,6+, ion irradiation also decreased in brain, lung and liver cells (,p,<,0.05). Nevertheless, no significant differences were observed in medium and higher doses groups irradiated and treated with glucosinolate by contrast with irradiation only group. The results show that the glucosinolate of lower doses can reduce the irradiation damages of mice induced by ,12,C,6+, ion beams, and related protecting mechanism needs further exploration.