The effects of electron beam irradiation on the sterilization and preservation of chilled pork were studied. The aim of this investigation was to provide academic and technical basis for application of electron beam irradiation on meat industry. The response surface analysis was used with electron beam energy(,X,1,) and dose(,X,2,) as factors and colony form unit(,Y,) as responses. The results have been shown that the model of sterilization of chilled pork by electron beam irradiation can be expressed ,Y,=3.78-0.24,X,1,-0.13,X,2,-0.16,X,1,X,2,-0.18,X,1,2,+0.15,X,1,2,(,R,2,=0.9755). It has been found there is a interaction between electron beam energy and absorbed doses, and the significance sequence of factors is absorbed dose,>,interaction,>, electron beam energy. When absorbed doses are in range from 3.23 kGy to 4.0 kGy and electron beam energy is in range from 2.3 MeV to 3.8 MeV, the colony form unit would drop 2 logarithm units. The shelf life of samples treated with electron beam irradiation is longer by about 12 d than that of control samples when the samples are stored at 4 ℃. When the samples are stored at 7~10 ℃, shelf life of samples treated with electron beam irradiation is longer by about 9 d than that of control samples. The results showed that electron beam irradiation has the effects of sterilization and preservation on chilled pork. This study has been confirmed that the application of electron beam irradiation is very useful for meat industry.