MA Shuang, LI Wenjian, WANG Jufang, et al. Studies on the effects of 12C6+ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1[J]. Journal of Radiation Research and Radiation Processing, 2009,27(2):125-128.
MA Shuang, LI Wenjian, WANG Jufang, et al. Studies on the effects of 12C6+ ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1[J]. Journal of Radiation Research and Radiation Processing, 2009,27(2):125-128.DOI:
Biocontrol bacterium BJ1 was irradiated with different doses of ,12,C,6+, ion beam in order to improve the antagonistic ability, to optimize the parameters of ion beam irradiation and to obtain high effect of biocontrol strains. The results show that the survival rate of BJ1 irradiated with 100 Gy reaches the highest and the survival curve of BJ1 presents a tendency of going down first and then increasing, which takes a shape of "saddle" during 0 to100 Gy. In view of the effect of mutation induction, BJ1 irradiated with 400 Gy enhances its effect of antagonistic ability obviously. Based on the results of survival rate and the effect of antagonistic ability, an optimal treatment dose of ,12,C,6+, irradiation is 200 to 400 Gy for BJ1. And BJ1 has been identified to be Bacillus subtilin by 16S rDNA sequence cluster analysis.
关键词
离子辐照生防菌BJ1诱变菌种鉴定
Keywords
Ion beam irradiationBiocontrol bacterium-BJ1MutationIdentification