Either internal radiation using radionuclide or external radiation using different rays induced bystander effects have been confirmed certainly by use of the cell cultured in vitro or in vivo exposed to radiation. There are many methods to study the bystander effects, such as conventional low-dose radiation, un-irradiated cells co-cultured with irradiated cells, treatment with irradiated cells conditioned media, single-cell micro-beam irradiation, and so on. Whichever method is used depends on the laboratory conditions and research purposes. A series of summary has been made in this paper.