In this study, we synthesized a fullerene derivative C60-Lys via direct reaction of fullerene(C,60,) and Lysine, whose molecular structure was identified by elemental analysis, infrared spectrum,1,H-NMR and ,13,C-NMR. It has been found that the chemical C,60,-Lys has little toxicity on human lymphoblast cell AHH-1. CCK-8 assay and AnnexinV/PI staining assay were performed to evaluate the cell survival rate and apoptosis after γ rays irradiation. The cell survival rate of C,60,-Lys treatment groups (≤1200 mg/L) shows no significant difference compared with the control group. The results show that pretreatment of cell with C,60,-Lys(≥400 mg/L) markedly increases the cell survival and decreases the cell apoptosis rate after irradiation exposure in a dose-dependant manner. These results can be suggested that C,60,-Lys has certain radioprotection effects to AHH-1 cell against radiation induced damages.