A new hydrogel film was prepared by use of poly (vinyl alcohol) (PVA), poly(ethylene oxide)(PEO) and chitosan under γ-rays irradiation. The influences of absorbed dose and the chitosan content on the properties of hydrogel film were investigated. The results showed that the gel fraction increased with the absorbed dose increasing, but the swelling ratio decreased. The gel fraction could reach 70% when the absorbed dose up to 21 kGy. The gel fraction, tensile strength and elongation of the hydrogel films reduced with the increment of absorbed dose when it was more than 21 kGy. With the increment of chitosan content, the gel fraction and tensile strength as well as elongation of the hydrogel films decreased, but the swelling ratio increased. The special functional groups of chitosan didn’t change after irradiation, and the antimicrobial activity would be retained. A new kind of hydrogel film was obtained.