DIAO Jinshan, WANG Li, CHEN Zhen, et al. Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N+ implantation[J]. Journal of Radiation Research and Radiation Processing, 2010,28(6):345-351.
DIAO Jinshan, WANG Li, CHEN Zhen, et al. Strain improvement and optimization for β-glucosidase production in Aspergillus niger by low-energy N+ implantation[J]. Journal of Radiation Research and Radiation Processing, 2010,28(6):345-351.DOI:
Low-energy N,+, implantation was employed to mutate ,Aspergillus niger, Au to enhance productivity of β-glucosidase. Effects of N,+, on strains, survival and mutation rate were studied. After several rounds of implantation, activity of β-glucosidase of the final mutant Au 0847 reached 13.75 U/mL, which is higher by 106.8% than that of original strain Au, and its heritability was stabilized. Activity of β-glucosidase of Au 0847 reached 30.53 U/mL after further fermentation condition optimization.