JIN Guanghui, XUE Lixiang, FU Shibo, et al. INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS3 CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS[J]. Journal of Radiation Research and Radiation Processing, 2001,19(4):307-310.
JIN Guanghui, XUE Lixiang, FU Shibo, et al. INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS3 CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS[J]. Journal of Radiation Research and Radiation Processing, 2001,19(4):307-310.DOI:
Changes of time course in apoptosis of HeLaS,3, cells following irradiation with different doses X rays were observed. The percentages of apoptotic cells were examined by both flow cytometry(FCM) and image cytometry (ICM) after the cells were stained by propidium iodine (PI) and Hoechst33342 (HO). It was founded that the percentages of apoptotic cells increased after irradiation with 0.5—4.0Gy X rays and the peak value emerged at the 12th hour after irradiation. In additon the values examined by both FCM and ICM were nearly same and the tendency was identical. So irradiation may induce apoptosis of HeLaS,3, cell lines.