8 experimental groups were conducted according to four factors two doses orthogonal layout [L8 (2,7,) ] in present investigation. 90 healthy male rabbits and 90 healthy male mice were equally divided into 8 experimental groups and control group. Exprimental animals were exposed to γ-radiation combined with benzene,toluene and CO vapbour for 2h per day,5 days per week for 8 weeks. Two doses of γ-radiation, benzene,toluene and CO were 0.0375 and 0.0075Gy/d (for rabbits, 0.0473 and 0. 0095Gy/d for mice) , 182±33 and 40±15mg/m,3, 407±68 and 90±39mg/m,3, as well as 278±18mg/m,3,respectively. The main results were showed as follows: (1) γ-radiation,benzene and toluene could all induce chromosome aberration,SCEs and micronuclei of lymphocytes in rabbits and chromosome aberrations of bone marrow cells in rabbits and mice,but those was no observed in carbon monoxide. (2) γ-radiation and benzene induced chromosome aberrations and micronuclei of lymphocytes in rabbits as well as chromosome aberrations of bone marrow cells in mice,γ-radiation and toluene induced chromosome aberrations of lymphocytes (in rabbits) and bone marrow cells (in mice) ,benzene and toluene induced chromosome aberations of lymphoytes (in rabbits) and bone marrow cells (in mice) . They showed all significantly interaction. (3) In combined effects,the yield of chromosome aberrations,SCEs and micronuclei induced by ① experimental groups which four factors are all high doses were highest,however, those by induced ⑧ experimental groups which four factors are all low doses also were higher than those of control group and there were statistical difference exdcept SCEs.