X射线照射对EL-4淋巴瘤细胞周期进程及细胞倍增时间的影响

鞠桂芝; 付士波; 刘建香; 刘树铮

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X射线照射对EL-4淋巴瘤细胞周期进程及细胞倍增时间的影响

更新时间：2023-04-18

- X射线照射对EL-4淋巴瘤细胞周期进程及细胞倍增时间的影响
- EFFECT OF X-RAYS ON CELL CYCLE PROGRESSION AND CELL DOUBLING TIME IN EL-4 LYMPHOMA CELLS
- 辐射研究与辐射工艺学报 1998年16卷第3期页码：157-160
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物实验室　长春　130021
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目
- DOI：
  中图分类号：
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鞠桂芝, 付士波, 刘建香, 等. X射线照射对EL-4淋巴瘤细胞周期进程及细胞倍增时间的影响[J]. 辐射研究与辐射工艺学报, 1998,16(3):157-160.

Ju Guizhi, Fu Shibo, Liu Jianxiang, et al. EFFECT OF X-RAYS ON CELL CYCLE PROGRESSION AND CELL DOUBLING TIME IN EL-4 LYMPHOMA CELLS[J]. Journal of Radiation Research and Radiation Processing, 1998,16(3):157-160.
鞠桂芝, 付士波, 刘建香, 等. X射线照射对EL-4淋巴瘤细胞周期进程及细胞倍增时间的影响[J]. 辐射研究与辐射工艺学报, 1998,16(3):157-160. DOI：

Ju Guizhi, Fu Shibo, Liu Jianxiang, et al. EFFECT OF X-RAYS ON CELL CYCLE PROGRESSION AND CELL DOUBLING TIME IN EL-4 LYMPHOMA CELLS[J]. Journal of Radiation Research and Radiation Processing, 1998,16(3):157-160. DOI：

摘要

目的研究不同剂量X射线照射对EL-4淋巴瘤细胞周期进程及细胞倍增时间的影响。方法离体培养EL-4细胞，计算细胞倍增时间。PI荧光探针标记，流式细胞术（FCM）检测细胞周期各时相细胞的变化。结果0.5Gy～4.0Gy X射线照射使EL-4细胞倍增时间明显延长，G,1,及G,2,期细胞数明显增加。结论0.5Gy～4.0Gy X射线照射诱导EL-4细胞明显的G,1,及G,2,期细胞阻滞，此变化显示出明显的剂量效应和时间效应。细胞周期进程变化与细胞倍增时间延长密切相关。

Abstract

In the present study,the effect of X-rays on EL-4 cell doubling time and cell cycle progression were investigated. Cell doubling time in culture was calculated. Propidium iodide (PI) staining and flow cytometer (FCM) were used for cell cycle measurement. The results showed that EL-4 cell doubling time was prolonged significantly after irradiation with doses of 0.5, 1.0,2.0 or 4.0Gy. G,1, arrest occurred at 72h after 1.0Gy and at 12h to 72h after 2.0 or 4.0Gy. G,2, arrest occurred at 72h after 0.5 or 1.0Gy and at 48h to 72h after 2.0 or 4.0Gy. S phase cells decreased after 1.0,2.0 or 4.0Gy exposure. We concluded that 0.5 to 4. 0Gy X-irradiation could induce G,1, and G,2, arrest for EL-4 cells,which related closely to cell doubling time prolongation.

关键词

电离辐射G1期阻滞G2期阻滞细胞倍增时间

Keywords

X-irradiationG1 arrestG2 arrestCell doubling time

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