晚期混合裂变产物内照射诱发体细胞HPRT基因位点突变的剂量效应关系研究

徐永忠; 赵经涌; 劳勤华

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晚期混合裂变产物内照射诱发体细胞HPRT基因位点突变的剂量效应关系研究

更新时间：2023-04-18

- 晚期混合裂变产物内照射诱发体细胞HPRT基因位点突变的剂量效应关系研究
- STUDY ON THE RELATIONSHIP BETWEEN ACCUMULATIVE DOSE AND SOMATIC CELL MUTATION AT HPRT LOCUS INDUCED BY LATE FISSION PRODUCTS
- 辐射研究与辐射工艺学报 1998年16卷第3期页码：165-168
- 作者机构：
  
  1.苏州医学院放射医学系　苏州　215007
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徐永忠, 赵经涌, 劳勤华. 晚期混合裂变产物内照射诱发体细胞HPRT基因位点突变的剂量效应关系研究[J]. 辐射研究与辐射工艺学报, 1998,16(3):165-168.

Xu Yongzhong, Zhao Jingyong, Lao Qinghua. STUDY ON THE RELATIONSHIP BETWEEN ACCUMULATIVE DOSE AND SOMATIC CELL MUTATION AT HPRT LOCUS INDUCED BY LATE FISSION PRODUCTS[J]. Journal of Radiation Research and Radiation Processing, 1998,16(3):165-168.
徐永忠, 赵经涌, 劳勤华. 晚期混合裂变产物内照射诱发体细胞HPRT基因位点突变的剂量效应关系研究[J]. 辐射研究与辐射工艺学报, 1998,16(3):165-168. DOI：

Xu Yongzhong, Zhao Jingyong, Lao Qinghua. STUDY ON THE RELATIONSHIP BETWEEN ACCUMULATIVE DOSE AND SOMATIC CELL MUTATION AT HPRT LOCUS INDUCED BY LATE FISSION PRODUCTS[J]. Journal of Radiation Research and Radiation Processing, 1998,16(3):165-168. DOI：

摘要

运用多核细胞法及胞质分裂阻断微核（CBMN）法对5组Wistar雄性大鼠外周血淋巴细胞HPRT基因位点突变频率及微核进行检测。实验组静脉注射放射性比活度为3.64×10,5, Bq/ml的晚期混合裂变产物后1至9 d心脏穿刺取血。结果表明：静注后第1d，累积剂量达1.73 cSv时，与对照组相比，HPRT位点突变频率、、微核率均已显著增加（,p,<,0.01）；累积剂量与HPRT位点突变频率、微核细胞率、微核率间均可拟合成剂量效应函数Y=a+b lnX; HPRT基因突变检测可望成为生物剂量计。

Abstract

With the technique of multinucleus cell and cytokinesis-block micronucleus (CBMN) method, the relationships between mutations at HPRT loci,micronuclei and accumulative doses were analyzed. The results were as follows: On the first day after intravenous injection (accumulative dose 1.73cSv) ,by comparison with the control group, the HPRT mutant frequencies,micronucleus cell frequencies and micronucleus frequencies increased significantly (p<0.01) . There existed a hemilogarithmic linear relationship between mutant frequencies at HPRT loci,micronucleus cell frequencies,micronucleus frequencies and accumulative doses, which could be described by following regression equation: Y=a+b lnX. A good linear relationship between HPRT mutant frequencies and micronucleus frequencies was observed. It is expected that the detection of HPRT locus mutation may soon be used as a biological dosimeter.

关键词

晚期混合裂变产物HPRT基因突变微核

Keywords

Late fission productsHPRT gene mutationMicronucleus

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