Poly （ethylene oxide） （PEO） hydrogel films formed by electron beam （EB） irradiation were prepared as wound dressing. The physiochemical properties of the PEO hydrogel films were evaluated. The results showed that properties of the PEO hydrogel films were largely dependent on irradiation dose,polymer concentration and its molecular weight. As the EB irradiation dose increased,the gel fraction and the hardness of the PEO hydrogel films also increased,while its degree of swelling, the tensile strength and elongation at break decreased dramatically. With the increase of the polymer concentration, the degree of swelling, the tensile strength and elongation at break of the PEO E-30 hydrogel films increased significantly,while their gel fraction and hardness decreased obviously. When the PEO molecular weight increased,the gel fraction,the degree of swelling and the hardness of the PEO hydrogel films increased obviously as well. The experimental results indicated that the PEO E-30 hydrogel film of 20 wt% possessed a better mechanical strength than other PEO hydrogel films,especially at doses of 40～60 kGy.