DNA聚合酶β在受照H<sub>22</sub>肝癌细胞核DNA修复中的作用

蒋逸风; 郑秀龙

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DNA聚合酶β在受照H₂₂肝癌细胞核DNA修复中的作用

更新时间：2023-04-23

- DNA聚合酶β在受照H₂₂肝癌细胞核DNA修复中的作用
- THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI
- 辐射研究与辐射工艺学报 1990年8卷第3期页码：150-154,144
- 作者机构：
  
  1.第二军医大学，上海
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助课题
- DOI：
  中图分类号：
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蒋逸风, 郑秀龙. DNA聚合酶β在受照H₂₂肝癌细胞核DNA修复中的作用[J]. 辐射研究与辐射工艺学报, 1990,8(3):150-154,144.

Jiang Yifeng, Zheng Xiulong. THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI[J]. Journal of Radiation Research and Radiation Processing, 1990,8(3):150-154,144.
蒋逸风, 郑秀龙. DNA聚合酶β在受照H₂₂肝癌细胞核DNA修复中的作用[J]. 辐射研究与辐射工艺学报, 1990,8(3):150-154,144. DOI：

Jiang Yifeng, Zheng Xiulong. THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI[J]. Journal of Radiation Research and Radiation Processing, 1990,8(3):150-154,144. DOI：

摘要

本文采用DNA聚合酶,α,或／和,β,酶专一性抑制剂（NEM或／和d,2,TTP），观察了小鼠腹水型H,22,肝癌细胞核DNA聚合酶α、,β,在40 Gy γ线照后DNA修复合成中的作用。结果表明：在α酶被完全抑制条件下，照射组在37℃保温60、120 min后DNA修复合成均明显高于未受照组（P,<,0.05、P,<, 0.01）；当,β,酶被完全抑制条件下，照射组与未受照组在37℃保温30、60和120 min后DNA修复合成无明显差别（,P,>,0.05）；当α及,β,酶均被抑制时，照射组与未受照组DNA修复合成亦无明显差别（P,>, 0.05）。上述结果证明了在本实验条件下，,β,酶参与了受γ线照射后肝癌细胞核DNA的修复作用，而α酶则不参与。作者并对上述结果进行了讨论。

Abstract

The effects of DNA polymerase alpha and beta on DNA repair of the 40 Gy γ-rays irradiated H,22, cell nuclei, using specific inhibitor NEM or ddTTP to DNA polymerase alpha or beta respectively, were observed. The results showed that the rate of DNA repair synthesis of the 40 Gy γ-rays irradiated H,22, cell nuclei was much higher than that of the non-irradiated ones after 60,120 min incubation at 37℃(P,<,0.05,P,<,0.01)when DNA polymerase alpha was inhibited completely. There was no difference in the rate of DNA repair synthesis between the irradiated and the non-irradiated cell nuclei (P,>,0.05) when DNA polymerase beta was inhibited completely. And also, there was no difference between them (P,>,0.05) when both DNA polymerase alpha and beta were inhibited completely. These results indicate that under our experimental conditions, DNA polymerase beta, not alpha, is responsible for DNA repair of the γ-rays irradiated H,22, cell nuclei. The above results are discussed by the author considerably.

关键词

DNA聚合酶αDNA聚合酶βDNA修复合成NEMd2TTP

Keywords

DNA polymerase alphaDNA polymerase beta DNA repairNEMddTTP

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DNA聚合酶β在受照H22肝癌细胞核DNA修复中的作用

THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H22) CELL NUCLEI

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摘要

Abstract

关键词

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DNA聚合酶β在受照H₂₂肝癌细胞核DNA修复中的作用

THE EFFECT OF DNA POLYMERASE BETA ON REPAIR OF THE γ-RAYS IRRADIATED HEPATOMA(H₂₂) CELL NUCLEI