Guo Jinhui, Ha Hongfei, Zhang Yuhua. STUDY ON IMMOBILIZATIONS OF OVINE ANTI-HUMAN IgGAND MCAb AGAINST EHF ON RADIATION-MODIFIED SILICONE FILMS[J]. Journal of Radiation Research and Radiation Processing, 1990,8(3):163-167.
Guo Jinhui, Ha Hongfei, Zhang Yuhua. STUDY ON IMMOBILIZATIONS OF OVINE ANTI-HUMAN IgGAND MCAb AGAINST EHF ON RADIATION-MODIFIED SILICONE FILMS[J]. Journal of Radiation Research and Radiation Processing, 1990,8(3):163-167.DOI:
In this work films of silicone (silastic) were grafted by monomer acrylamide vis γ-radiation technology and then the ovine anti-human IgG, Epidemic hemorrhagic fever (EHF)-MCAb were immobilized on the silastic-AAM films with different grafting yields passthrough associate reactions. Measurements of relationships between grafting yields. contents of immobilized antibodies and immunoactivities for immobilized silastic-AAM films were performed by using ,125,I method ELISA method was used to measure the immunoactivities for the immobilized monoantibody. The results showed that the antibodies used can be immobilized on radiation-grafted silicone films and this immobilization method has its potential significance in clinical practice.