刀豆蛋白A诱导的CD4+和CD8+细胞调节功能及其辐射效应的研究

许玉杰; 苏燎原; 刘克良

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刀豆蛋白A诱导的CD₄⁺和CD₈⁺细胞调节功能及其辐射效应的研究

更新时间：2023-04-23

- 刀豆蛋白A诱导的CD₄⁺和CD₈⁺细胞调节功能及其辐射效应的研究
- MODULATION FUNCTION AND EFFECT OF ⁶⁰Co γ RAYS ON CONCANAVALIN A (ConA)-INDUCED CD₄+ AND CD₈+ SUBPOPULATIONS OF LYMPHOCYTE
- 辐射研究与辐射工艺学报 1992年10卷第2期页码：81-86
- 作者机构：
  
  1.苏州医学院，苏州
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许玉杰, 苏燎原, 刘克良. 刀豆蛋白A诱导的CD₄⁺和CD₈⁺细胞调节功能及其辐射效应的研究[J]. 辐射研究与辐射工艺学报, 1992,10(2):81-86.

Xu Yujie, Su Liaoyuan, Liu Keliang. MODULATION FUNCTION AND EFFECT OF ⁶⁰Co γ RAYS ON CONCANAVALIN A (ConA)-INDUCED CD₄+ AND CD₈+ SUBPOPULATIONS OF LYMPHOCYTE[J]. Journal of Radiation Research and Radiation Processing, 1992,10(2):81-86.
许玉杰, 苏燎原, 刘克良. 刀豆蛋白A诱导的CD₄⁺和CD₈⁺细胞调节功能及其辐射效应的研究[J]. 辐射研究与辐射工艺学报, 1992,10(2):81-86. DOI：

Xu Yujie, Su Liaoyuan, Liu Keliang. MODULATION FUNCTION AND EFFECT OF ⁶⁰Co γ RAYS ON CONCANAVALIN A (ConA)-INDUCED CD₄+ AND CD₈+ SUBPOPULATIONS OF LYMPHOCYTE[J]. Journal of Radiation Research and Radiation Processing, 1992,10(2):81-86. DOI：

摘要

使用单克隆抗体CD,4,和CD,8,，采用Panning法分离并获得B、CD,4,+,和CD,8,+,细胞亚群，间接免疫荧光试验鉴定获得的细胞纯度分别是82.7%、90.4%和90.8%；台盼蓝拒染法测定细胞活性，三者均达92%以上。用LPS诱导的B淋巴细胞，刀豆蛋白A（ConA）诱导的CD,4,+,、CD,8,+,细胞，以及经过10 Gy ,60,Coγ线照射的ConA诱导的CD,4,+,和CD,8,+,细胞，进行匹配分组培养，,3,HTdR参入法观察ConA CD,4,+,和ConA CD,8,+,细胞的抑制功能及其辐射效应。发现ConA CD,4,+,和ConA CD,8,+,细胞都有抑制LPS诱导的B淋巴细胞转化的功能，10 Gy的γ射线照射对两者的抑制功能没有影响。ConA CD,4,+,细胞可以增强ConA CD,8,+,细胞的抑制功能；反之，ConA CD,8,+,细胞也可以增强ConA CD,4,+,细胞的抑制功能；10 Gy γ射线照射使两者的增强效应消失。

Abstract

The monocyte depleted peripheral blood mononuclear cells were saparated into B, CD,4,+, and CD,8,+, subpopulations by a Panning technique with McAb CD,4, and CD,8,. The purity of the B, CD,4,+, and CD,8,+, subpopulations was 82.7%, 90.4%and 90.8% respectively, which was estimated by an indirect immunofluorescence technique. More than 92% of the three subpopulations were viable according to the trypan blue exclusion test. The modulation function of ConA-induced CD,4,+, and CD,8,+, cells and the effect of 10 Gy ,60,Co γ rays on them were investigated by the ,3,HTdR incorporation method. It demonstrated that the transformation of LPS-induced B lymphocytes was suppressed by the ConA-induced CD,4,+, and CD,8,+, cells. After irradiation, the suppressor activity of ConA-induced CD,4,+, and CD,8,+, cells did not decrease. It also showed that the suppressor activity ,of, ConA-induced CD,8,+, cells was enhanced by the ConA-induced CD,4,+, cells significantly, and the suppressor activity of ConA-induced CD,4,+, cells was also enhanced by the ConA-induced CD,8,+, cells. After irradiation, the enhancement effects of the ConA-induced CD,4,+, and CD,8,+, cells disappeared.

关键词

刀豆蛋白A（ConA）单克隆抗体（McAb）淋巴细胞亚群3HTdR辐射效应

Keywords

Concanavalin AMonoclonal antibodyLymphocyte subpopulation3HTdRRadiation Effect

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