<italic style="font-style: italic">γ</italic>射线诱导的哺乳动物细胞基因突变

季守平; 章扬培

您当前的位置：

首页 >

文章列表页 >

γ射线诱导的哺乳动物细胞基因突变

更新时间：2023-04-23

- γ射线诱导的哺乳动物细胞基因突变
- GENE MUTATION INDUCED BY γ- IRRADIATION IN MAMMALIAN CELLS
- 辐射研究与辐射工艺学报 1994年12卷第1期页码：7-10
- 作者机构：
  
  1.军事医学科学院放射医学研究所，北京　100850
- 作者简介：
- 基金信息：
  
  国家自然科学基金重点及面上资助项目
- DOI：
  中图分类号：
扫描看全文
季守平, 章扬培. γ射线诱导的哺乳动物细胞基因突变[J]. 辐射研究与辐射工艺学报, 1994,12(1):7-10.

Ji Shouping, Zhang Yangpei. GENE MUTATION INDUCED BY γ- IRRADIATION IN MAMMALIAN CELLS[J]. Journal of Radiation Research and Radiation Processing, 1994,12(1):7-10.
季守平, 章扬培. γ射线诱导的哺乳动物细胞基因突变[J]. 辐射研究与辐射工艺学报, 1994,12(1):7-10. DOI：

Ji Shouping, Zhang Yangpei. GENE MUTATION INDUCED BY γ- IRRADIATION IN MAMMALIAN CELLS[J]. Journal of Radiation Research and Radiation Processing, 1994,12(1):7-10. DOI：

摘要

观察了电离辐射对中国仓鼠细胞CHO-K,1,和人细胞HeLa MR的非必需基因hprt和必需基因Na,+,/K,+,ATP酶的基因致突能力。γ射线能增加两种细胞hprt基因的突变频率，照射剂量和突变频率呈正相关，在相同的照射剂量下CHO-K,1,的突变频率高于HeLa MR，提示在hprt位点，鼠细胞对辐射的敏感性比人细胞高。在Na,+,/K+ATP酶基因位点，辐射不能增加两种细胞的突变频率。HPRT和Na,+,/K,+,ATP酶活分析证实了实验的可靠性。

Abstract

CHO-K,1, and HeLa MR cells were irradiated with ,60,Co γ-ray, the mutagenesis of one nonessential gene (hprt) and an essential gene (Na,+,/K,+, ATPase gene) have been observed. Enzyme assay confirmed the results of mutation induction. At hprt locus, γ-irradiation increased the mutation frequency of both cell lines and there was a linear relationship between mutation frequency and exposure dose. The mutagenecity of CHO-K,1, was higher than that of HeLa MR cells. The results indicated that hprt gene was more susceptible to mutagenesis in hamster cells than that in human cells induced by γ-ray. No induced mutation was found in both CHO-K,1, and HeLa MR cells at Na,+,/K,+, ATP ase gene locus.

关键词

γ辐射哺乳动物细胞基因突变辐射细胞效应酶

Keywords

γ-radiationMammalian cellGene mutationCellular radiation effectsEnzyme

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

α粒子照射诱发细胞基因突变的旁效应及机理研究

辐射诱导造血祖细胞（GM-CFU）的HPRT基因突变

⁶⁰Co γ辐照对胰酶活力影响的研究

γ射线所致哺乳动物细胞hprt基因突变分子机理的初步探讨

γ射线诱导的哺乳动物细胞基因突变

GENE MUTATION INDUCED BY γ- IRRADIATION IN MAMMALIAN CELLS

DOI：

摘要

Abstract

关键词

Keywords

references