混输脾基质细胞与骨髓细胞悬液在小鼠大剂量辐射损伤修复中的作用

于洪臣; 凌翎; 李勇; 张英姬; 孟洪旂; 王跃宇; 张红梅; 刘及

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混输脾基质细胞与骨髓细胞悬液在小鼠大剂量辐射损伤修复中的作用

更新时间：2023-04-23

- 混输脾基质细胞与骨髓细胞悬液在小鼠大剂量辐射损伤修复中的作用
- THE EFFECT OF MIXED INJECTION OF SPLENIC STROMAL CELL SUSPENSION AND BONE MARROW CELLS ON THE RECOVERY OF LARGE DOSE RADIATION INJURY IN MICE
- 辐射研究与辐射工艺学报 1994年12卷第1期页码：40-43
- 作者机构：
  
  1.白求恩医科大学预防医学院，长春　130021
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助课题(39070315)
- DOI：
  中图分类号：
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于洪臣, 凌翎, 李勇, 等. 混输脾基质细胞与骨髓细胞悬液在小鼠大剂量辐射损伤修复中的作用[J]. 辐射研究与辐射工艺学报, 1994,12(1):40-43.

Yu Hongchen, Ling Ling, Li Yiong, et al. THE EFFECT OF MIXED INJECTION OF SPLENIC STROMAL CELL SUSPENSION AND BONE MARROW CELLS ON THE RECOVERY OF LARGE DOSE RADIATION INJURY IN MICE[J]. Journal of Radiation Research and Radiation Processing, 1994,12(1):40-43.
于洪臣, 凌翎, 李勇, 等. 混输脾基质细胞与骨髓细胞悬液在小鼠大剂量辐射损伤修复中的作用[J]. 辐射研究与辐射工艺学报, 1994,12(1):40-43. DOI：

Yu Hongchen, Ling Ling, Li Yiong, et al. THE EFFECT OF MIXED INJECTION OF SPLENIC STROMAL CELL SUSPENSION AND BONE MARROW CELLS ON THE RECOVERY OF LARGE DOSE RADIATION INJURY IN MICE[J]. Journal of Radiation Research and Radiation Processing, 1994,12(1):40-43. DOI：

摘要

C,57,BL/6小鼠经,60,Co γ射线一次整体照射8 Gy后，分成三组：（1）单纯照射8 Gy组，（2）照射后单纯骨髓移植组，（3）照射后混输脾基质细胞（SSC）与骨髓细胞（BMC）组，以观察骨髓移植和回输脾基质细胞对大剂量照射后受体存活率及造血干细胞（CFU-S）的影响，并初步探讨其可能的机理。结果表明：单纯骨髓移植组受体存活率比单纯照射组高出27%；而照射后混输SSC组之存活率比单照射组高47%，比单纯骨髓移植组高22%（P,<,0.05）。混输SSC可明显提高脾CFU-S、CFU-F及ANAE染色,（+）（-）,细胞数。在体外混合培养中脾基质细胞对实质细胞增殖起支持与调节作用。

Abstract

The paper reported that C,57, BL/6 mice after exposure to 8Gy γ-rays irradiation were divided into three groups: (1) the control group (8 Gy only),(2) the group with bone marrow cell (BMC) injection only,(3) the group with mixed injection of BMC and splenic stromal cell (SSC) which cultured in order to observe the effect of bone marrow transplantation and injection of SSC on the survival rate of recipients and on the number of hemopoietic stem cells (CFU-S) after large dose irradiation and study its mechanism. The results of experiments suggested that the survival rate of recipients of the group with BM cells injection only was 27% higher than that of the control group, but that of the group with SSC mixed injection was 49% higher than that of the control group and 22% higher than that of the group with BMC injection only. The CFU-S shows that mixed SSC injection improve the number of CFU-S,CFU-F and ANAE,[+][-], lymphocyte in spleen significantly. The cocultivation of spleenocytes and SSC in vitro showed the spleen stromal cells surport and regulate their parenchyma cells.

关键词

脾基质细胞大剂量照射骨髓移植存活率

Keywords

Spleen stromal cellsLarge dose irradiationBone marrow transplantationSurvival rate

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