The imature embryo callus of wheat were radiated by γ-rays of different doses. When the dose was below 5 Gy, the relative growth rate of callus increased, the aberration frequency of callus and chromosomes were very low. When the dose was above 15 Gy, the aberration frequency of cells and chromosomes increased significantly, but the growth rate of callus decreased significantly, thus much callus grew so slowly and died. When the dose was 10 Gy, the aberration frequency of cells and chromosomes were not very low, and the growth of callus was not greatly inhibited. So, 10 Gy may be regarded as a suitable radiation dose for wheat imature embryo callus.