人食道癌细胞系CaEs-17放射敏感性与H-ras的扩增

王顺宝; 郭蕾; 牛文哲

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人食道癌细胞系CaEs-17放射敏感性与H-ras的扩增

简报 | 更新时间：2023-04-23

- 人食道癌细胞系CaEs-17放射敏感性与H-ras的扩增
- HETEROGENEITY IN RADIOSENSITIVITY WITHIN ESOPHAGEAL CELL LINE CaEs-17 AND AMPLIFICATION OF H-ras GENE
- 辐射研究与辐射工艺学报 1997年15卷第1期页码：60-62
- 作者机构：
  
  1.中国医学科学院肿瘤研究所|中国协和医科大学　肿瘤医院放射生物室　北京　100021
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王顺宝, 郭蕾, 牛文哲. 人食道癌细胞系CaEs-17放射敏感性与H-ras的扩增[J]. 辐射研究与辐射工艺学报, 1997,15(1):60-62.

Wang Shunbao, Guo Lei, Niu Wenzhe. HETEROGENEITY IN RADIOSENSITIVITY WITHIN ESOPHAGEAL CELL LINE CaEs-17 AND AMPLIFICATION OF H-ras GENE[J]. Journal of Radiation Research and Radiation Processing, 1997,15(1):60-62.
王顺宝, 郭蕾, 牛文哲. 人食道癌细胞系CaEs-17放射敏感性与H-ras的扩增[J]. 辐射研究与辐射工艺学报, 1997,15(1):60-62. DOI：

Wang Shunbao, Guo Lei, Niu Wenzhe. HETEROGENEITY IN RADIOSENSITIVITY WITHIN ESOPHAGEAL CELL LINE CaEs-17 AND AMPLIFICATION OF H-ras GENE[J]. Journal of Radiation Research and Radiation Processing, 1997,15(1):60-62. DOI：

摘要

从我国自行建株的人食道癌细胞系CaEs-17野生型中分离出两株放射抗拒的亚系克隆7和克隆10,其SF,2,值分别为0.41、0.58和0.61。存活曲线的分析表明，与野生型比较，克隆7及克隆10的D,0,值差异不大，但D,Q,与N值有较大的差别。野生型细胞系D,Q,为1.07 Gy，而克隆7的D,Q,为1.85 Gy,克隆10的D,Q,值为2.04Gy；野生型细胞的N值为1.96,克隆7亚系的N值为3.07,克隆10亚系的N值为3.63。用Southern Blot杂交检测了H-ras基因的扩增，结果发现克隆10亚系在照射2 Gy后H-ras显著扩增，可能克隆10的放射抗拒与H-ras的扩增有一定关系，但未发现两者之间有恒定的关系。

Abstract

Ten clones were picked from cultured colonies of CaEs-17 cell line and developed to a subcell line. The values of SF,2, were measured for each subcell line, Two radioresistant subcell lines, clone 7 and clone 10 were established,According to survival curve assay,for wild type, the value of D,0, was 1,.,57 Gy. D,Q, was 1.07 Gy, N was 1.96, SF,2, was 0.41. For clone 7,the value of D,0, was 1.64 Gy, D,Q, was 1.85 Gy, N was 3.07, SF,2, was 0.53. For clone 10, the value of D,0, was 1.58 Gy, D,Q, was 2,04 Gy, N was 3.63, SF,2, was 0.61. Clone 7 and clone 10 have much higher values of D,Q, and N than those of wild type, there was amplification of H-ras gene in clone 10 after 2 Gy irradiation. The amplification of H-ras gene in clone 10 after 2 Gy irradiation might be involved in hetero geneity of CaEs-17 cell line.

关键词

放射敏感性人食道癌细胞系存活曲线H-ras癌基因

Keywords

RadiosensitivityH-raa geneSurvival curveHuman esophageal carcinoma cell line

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