p53和Bcl - 2/Bax在辐射诱导胸腺细胞凋亡中的作用

苏旭; 万虹; 刘树铮

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p53和Bcl - 2/Bax在辐射诱导胸腺细胞凋亡中的作用

研究论文 | 更新时间：2023-04-23

- p53和Bcl - 2/Bax在辐射诱导胸腺细胞凋亡中的作用
- THE EFFECTS OF p53 AND Bcl — 2/Bax ON APOPTOSIS OF THYMOCYTES INDUCED BY IONIZING RADIATION
- 辐射研究与辐射工艺学报 1997年15卷第2期页码：119-122
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物重点实验室，长春　130026
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苏旭, 万虹, 刘树铮. p53和Bcl - 2/Bax在辐射诱导胸腺细胞凋亡中的作用[J]. 辐射研究与辐射工艺学报, 1997,15(2):119-122.

Su Xu, Wan Hong, Lui Shuzheng. THE EFFECTS OF p53 AND Bcl — 2/Bax ON APOPTOSIS OF THYMOCYTES INDUCED BY IONIZING RADIATION[J]. Journal of Radiation Research and Radiation Processing, 1997,15(2):119-122.
苏旭, 万虹, 刘树铮. p53和Bcl - 2/Bax在辐射诱导胸腺细胞凋亡中的作用[J]. 辐射研究与辐射工艺学报, 1997,15(2):119-122. DOI：

Su Xu, Wan Hong, Lui Shuzheng. THE EFFECTS OF p53 AND Bcl — 2/Bax ON APOPTOSIS OF THYMOCYTES INDUCED BY IONIZING RADIATION[J]. Journal of Radiation Research and Radiation Processing, 1997,15(2):119-122. DOI：

摘要

采用流式细胞术对昆明小鼠接受75mGy和2Gy X射线全身照射后，胸腺细胞内细胞凋亡相关基因p53、Bcl-2和Bax蛋白的表达进行了比较研究。结果表明，75mGy X射线全身照射后24h,p53基因蛋白产物的表达显著降低（,P,<,0.005）,而2Gy X射线全身照射后24h,p53基因蛋白表达则显著增高（,P,<,0.005）。75mGy X射线全身照射后24h,Bcl-2/Bax比值明显增加（,P,<,0.05）。而2Gy X射线全身照射组，Bcl — 2/Bax比值无明显变化。提示：p53和Bcl-2/Bax在辐射诱导的胸腺细胞凋亡中起着重要作用。

Abstract

Kunming mice were whole-body irradiated (WBI) with 75mGy and 2Gy X-rays. The expression of p53, Bcl-2 and Bax proteinswas analyzed with flow cytometry. It was found that the expression ofp53 protein showed,significantly decreased (,p,<,0.005) 24 hours after WBI with 75mGy X-rays and significantly increased ,(p,<,0.005) after WBIwith 2 Gy X-rays. The ratio of Bcl-2/Bax was significantly increased(,p,<,0.05) 24 hours after WBI with 75mGy X-rays and not significant changed after WBI with 2Gy. The change in expression of p53 and Bcl-2/Baxratio WBI with 75mGy and 2Gy X — rays may give partial explanation to the inhibitory effect of low dose radiation on thymocyte apoptosis. The results suggested that p53 and Bcl-2/Bax play an important role on apoptosis of thymocytes induced by ionizing radiation.

关键词

p53Bcl-2Bax细胞凋亡电离辐射流式细胞术（FCM）

Keywords

p53Bcl-2BaxApoptosisIonizing radiationFlow cytometry(FCM)

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