To investigate the effects of chemically modi?ed tetracycline 8 (CMT-8) on cell growth, migration and radio-senstivity in human cervical cancer HeLa cells, MTT assay was used to determine cell viability and radiosensitivity; wound healing and adhesive assays were used to measure cell migration and adhesion; flow cytometry and Western blot assay were performed to analyze cell cycle alteration and protein expression. The results showed that CMT-8 inhibited HeLa cell growth, migration and adhesion in a dose-and time dependent fashion. Furthermore, CMT-8 caused a G0/G1 arrest, in accompany with a dose-dependent down-regulation of Cyclin D and Cyclin E protein expression. Finally, HeLa cells pre-treated with CMT-8 at non-cytotoxic concentrations became more sensitive to subsequent irradiation of X-rays, which was related to a decreased expression of Ku70 and DNA-PKcs protein. Taken together, these findings indicate that CMT-8 is a new anti-cervical cancer compound via inhibiting cell growth and migration as well as mediating radio-sensitivity.Cited