The relationships between doses–survival rate and doses–T-AOC of Blakeslea trispora and Trichoderma reesei by N+ ion implantation were investigated. The results showed that the curves of survival rate of T.reesei, Streptomyces hygroscupicus var. Jingganggensis Yen. and Bacillus coagulans were all represented “double saddle shape” with increasing N+ implanted dose, which were different from the classic “saddle shape” curves. And the dose–T-AOC curves of B.trispora and T.reesei revealed the same trend as the dose–survival rate ones. It can be suggested that the variation of T-AOC probably decided the microbe survival situation after being implanted by low-energy N+ ion.Cited