In this work, micron-scaled sulfonated crosslinked polystyrene (SCPS) microspheres were prepared, and dispersed in water/styrene binary mixture as seed microspheres. The system was irradiated by 60Co g-rays after single-body swelled, and SCPS microspheres with grooved surface were fabricated. Two parameters, the content of crosslinking agent (DVB) and the sulfonation degree of the original SCPS seed microspheres, which influenced the final microspheres facial morphology were studied. The results show that walnut-like microspheres would be formed when the above two parameters are low, and plum-like microspheres prevail with increase of these two parameters. However, the special facial morphology has nothing to do with a certain dose rate range at 44−108 Gy•min−1. This work provides a facile and effective way to prepare polymer microspheres with roughness surface.Cited