Circular dichroism (CD) spectroscopy is one of the powerful tools for DNA structure research. Currently, the general CD spectrometer can only obtain the CD signal with wavelength above 190 nm, and the noise is very high with the wavelength range of 190−220 nm because of the influences from the air, the solvents, and the salt ion concentration of the buffer, etc., making it have not much practical value. The development of the synchrotron radiation circular dichroism (SRCD) makes it possible to obtain the CD signal of the DNA structure in the region of the vacuum ultraviolet (VUV). And now it becomes a new powerful tool for studying the DNA structure. This work firstly introduces the principle and features of SRCD technique, especially its advantages in the DNA structure research. Then the DNA structures and the factors affecting the CD signal are also reviewed. Finally, some typical DNA structures are taken as samples to analyze their SRCD spectrum.Cited