1. 1(华东师范大学物理系生物物理所 ),上海,200241
2. 2(华东师范大学精密光谱科学与技术国家重点实验室 ),上海,200241
[ "林锦文,女,1990年12月出生,2012年毕业于湖北文理学院,生物物理专业,目前为在读硕士研究生,E-mail: jewel092@163.com" ]
[ "王向晖,博士,副教授 E-mail:xhwang@phy.ecnu.edu.cn" ]
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林锦文, 刘 合, 张 杰, 等. 电磁脉冲对大鼠心脏和肝脏损伤及修复的影响[J]. 辐射研究与辐射工艺学报, 2015,33(6):60201-060201.
林锦文, 刘 合, 张 杰, 等. 电磁脉冲对大鼠心脏和肝脏损伤及修复的影响[J]. 辐射研究与辐射工艺学报, 2015,33(6):60201-060201. DOI: 10.11889/j.1000-3436.2015.rrj.33.060201.
采用宽频谱电磁脉冲(场强为110 kV/m和140 kV/m,脉冲个数为5个和100个,脉冲前沿20 ns,脉宽200 ns)辐照雄性大鼠,分别于受照后6 h、1 d、3 d、7 d、10 d和15 d取样,通过全自动生化分析仪和酶联免疫吸附试验(Elisa)法检测心、肝功能有关的血清生化指标(谷丙转氨酶ALT和谷草转氨酶AST),以及肝脏和心脏组织细胞凋亡相关蛋白(促凋亡蛋白Bax和抑凋亡蛋白Bcl-2)水平随辐照后时间而产生的变化,并表征大鼠心脏与肝脏组织受到的损伤以及随后的自我修复情况。结果表明:宽频谱电磁脉冲辐照后6 h大鼠肝功能相关血清ALT和AST明显降低,肝脏组织中Bax和Bcl-2表达水平均显著性升高,照后ALT、AST和Bax于1 d后、Bcl-2 于7 d后恢复到Sham组水平,说明辐照对大鼠肝脏组织产生了早期损伤效应并启动自我修复功能,1 d后得以修复;未观察到大鼠心脏组织的自我修复情况,推测辐照对其无显著性的早期损伤效应。
The influences of W-EMP (Wide-band electromagnetic pulse) exposure (field strength: 110 kV/m or 140 kV/m, pulse number: 5 or 100, pulse leading edge width: 20 ns, and the pulse width: 200 ns) on the liver and heart tissue of male rat, as well as subsequent recovery behavior, were investigated. At 6 h, 1 d, 3 d, 7 d, and 10 d separately from after-exposure, samples were collected for following assays to characterize the early damage and self-recovery of tissues: serum biochemical indexes (ALT and AST) related to heart and liver were detected by automatic biochemical analyzer, and the content of apoptosis-related proteins (Bax and Bcl-2) in both liver and heart was measured by Enzyme linked immunosorbent assay (Elisa). The results showed that significantly decreased levels of serum biochemical indicators (ALT and AST) and elevated apoptosis-related proteins (Bax and Bcl-2) (p<0.05) in liver were detected at 6 h of after-exposure, and recovered to Sham level 1 d (ALT, AST, Bax) and 7 d (Bcl-2) later, which implied that the liver of rat was early damaged by W-EMP exposure and subsequently activated the self-recovery response. However, no significant changes were induced by the W-EMP in the heart tissues, so no self-recovery behavior was available for observation.Cited
宽频谱电磁脉冲血清生化指标凋亡相关蛋白修复
Wide-band electromagnetic pulseSerum biochemical indexesApoptosis-related proteinsRecovery
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