线粒体靶向抗氧化剂MitoQ的抗肿瘤效应

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线粒体靶向抗氧化剂MitoQ的抗肿瘤效应

更新时间：2021-12-10

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- 线粒体靶向抗氧化剂MitoQ的抗肿瘤效应
- Anti-tumor effects of mitochondriatargeted antioxidant MitoQ
- 辐射研究与辐射工艺学报 2016年34卷第1期页码：10201
- 作者机构：
  
  1.中国医学科学院北京协和医学院放射医学研究所天津分子核医学重点实验室天津 300192
  2.Tianjin Key Laboratoryof Molecular Nuclear Medicine,Perking Union Medicine College &Chinese Academy of Medical Sciences,Institute of Radiation Medicine,Tianjin300192,China
- 作者简介：
  
  王华伟，男，1988 年9 月出生，2012 年毕业于河南大学，现为中国医学科学院放射医学研究所硕士研究生
  徐文清，研究员，硕士生导师，E-mail: xuwenqing@irm-cams.ac.cn
- 基金信息：
  
  天津市应用基础与前沿技术(14JCZDJC36400)、北京协和医学院青年基金(33320140124)和中国医学科学院放射医学研究所探索基金(1555)资助
- DOI：10.11889/j.1000-3436.2016.rrj.34.010201
  中图分类号：
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王华伟, 王华南, 康肖梦, 等. 线粒体靶向抗氧化剂MitoQ的抗肿瘤效应[J]. 辐射研究与辐射工艺学报, 2016,34(1):10201.

Huawei WANG, Huanan WANG, Xiaomeng KANG, et al. Anti-tumor effects of mitochondriatargeted antioxidant MitoQ[J]. Journal of Radiation Research and Radiation Processing, 2016,34(1):10201.
王华伟, 王华南, 康肖梦, 等. 线粒体靶向抗氧化剂MitoQ的抗肿瘤效应[J]. 辐射研究与辐射工艺学报, 2016,34(1):10201. DOI： 10.11889/j.1000-3436.2016.rrj.34.010201.

Huawei WANG, Huanan WANG, Xiaomeng KANG, et al. Anti-tumor effects of mitochondriatargeted antioxidant MitoQ[J]. Journal of Radiation Research and Radiation Processing, 2016,34(1):10201. DOI： 10.11889/j.1000-3436.2016.rrj.34.010201.

摘要

摘要,采用噻唑蓝(MTT)法检测不同浓度MitoQ对肝癌HepG2细胞的毒性；凋亡诱导实验研究不同浓度MitoQ对HepG2细胞凋亡率的影响；裸鼠移植瘤模型研究MitoQ对肝癌HepG2荷瘤小鼠的抑瘤作用。结果表明，MitoQ对肝癌HepG2细胞的抑制作用随着浓度而增加；给药组凋亡率显著高于对照组(,p,<,0.01)；MitoQ在1、5、10 mg/kg 剂量给药10 d后对小鼠的肿瘤生长均有抑制作用，与对照组相比，10 mg/kg剂量组肿瘤体积(,p,<,0.01)和肿瘤质量(,p,<,0.05)均有显著性差异。结果提示，MitoQ在体内体外均具有抑制肝癌HepG2细胞生长的作用。

Abstract

ABSTRACT, MTT was used to detect the celltoxicity of different concentrations of MitoQ on liver cancer HepG2 cells. Apoptosis-inducing assay was used todetect the apoptosis rate onliver cancer HepG2 cells after the treatment with varying MitoQ concentrations. Xenograft tumor model assay was used to investigate the anti-tumoreffects of MitoQ on HepG2 hepatocarcinoma-bearing mice. The results showed thatthe inhibitory effects ofMitoQ on HepG2 cells increased with MitoQ concentration,and compared with control group,the administration group apoptosis rate was significantly higher (,p,<,0.01). In nude mice xenograft tumor modelassay,the tumor growth wasall inhibited after the treatment with 1 mg/kg,5 mg/kg,and 10 mg/kg MitoQ concentration after 10 d. When the mice were treated with 10 mg/kg MitoQ,tumor volume (,p,<,0.01) and tumor mass(,p,<,0.05) were significantly different from those of the blank group (,p,<,0.01). The results indicate that MitoQ has antitumor effect ,in vivo, and ,in vitro,.

关键词

MitoQ抗肿瘤HepG2细胞细胞凋亡线粒体靶向

Keywords

MitoQAuti-tumorHepG2 cellCell apoptosisMitochondria targeted

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