碳离子束辐照选育截短侧耳素菌原生质体

都雯玥; 陆锡宏; 李雪虎; 周翔; 梁剑平; 辛志君

doi:10.11889/j.1000-3436.2016.rrj.34.010402

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碳离子束辐照选育截短侧耳素菌原生质体

更新时间：2021-12-10

- 碳离子束辐照选育截短侧耳素菌原生质体
- Breeding pleuromutilin protoplasts strains by carbon ion beam irradiation
- 辐射研究与辐射工艺学报 2016年34卷第1期页码：10402
- 作者机构：
  
  1.（中国科学院近代物理研究所兰州 730000）
  2.（兰州理工大学生命科学与工程学院兰州 730000）
- 作者简介：
  
  都雯玥，女，1988年6月出生，2011年毕业于甘肃农业大学，现为兰州理工大学在读硕士研究生，方向为微生物诱变育种
  李雪虎，博士，副研究员，E-mail: lixh@impcas.ac.cn
- 基金信息：
  
  国家高技术研究发展计划（863 计划）(2011AA10A214)和甘肃省陇药产业发展专项(Y361010SJ0)项目资助 Supported by National High Technology Research and Development Program of China (863Program) (2011AA10A214) and GansuLocal Pharmaceutical Industry Development Fund (Y361010SJ0)
- DOI：10.11889/j.1000-3436.2016.rrj.34.010402
  中图分类号：
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都雯玥, 陆锡宏, 李雪虎, 等. 碳离子束辐照选育截短侧耳素菌原生质体[J]. 辐射研究与辐射工艺学报, 2016,34(1):10402.

Wenyue DU, Xihong LU, Xuehu LI, et al. Breeding pleuromutilin protoplasts strains by carbon ion beam irradiation[J]. Journal of Radiation Research and Radiation Processing, 2016,34(1):10402.
都雯玥, 陆锡宏, 李雪虎, 等. 碳离子束辐照选育截短侧耳素菌原生质体[J]. 辐射研究与辐射工艺学报, 2016,34(1):10402. DOI： 10.11889/j.1000-3436.2016.rrj.34.010402.

Wenyue DU, Xihong LU, Xuehu LI, et al. Breeding pleuromutilin protoplasts strains by carbon ion beam irradiation[J]. Journal of Radiation Research and Radiation Processing, 2016,34(1):10402. DOI： 10.11889/j.1000-3436.2016.rrj.34.010402.

摘要

使用,12,C,6+,离子束辐照,Clitopilus pinsitus, 原生质体至不同吸收剂量，运用琼脂柱预筛和96孔板固体发酵选育截短侧耳素高产变异株。结果显示，最佳,12,C,6+,离子束吸收剂量为1.5 Gy，在该吸收剂量下正变异率为37.58%。选育出C.pin15I5E和C.pin15II6B两株正变异株，其产量较出发菌株分别提高16.17%和15.47%。表明重离子束辐照原生质体是行之有效的工业微生物诱变育种方法。

Abstract

Clitopilus pinsitus, protoplast was irradiated by ,12,C,6+, ion beam at varied absorbed doses for mutagenesis,and high-yielding pleuromutilin mutant strains were screened using high-throughput method and solid fermentation. The results showed that the optimum ,12,C,6+, ion beam absorbed dose is 1.5 Gy,and under this condition the positive mutation rate is 37.58%. By agar column prescreening and 96-plate solid fermentation,the mutant strains,C.pin15I5E and C.pin15II6B,were selected. The yields of the strain increased by 16.17% and 15.47%,respectively,compared with that of the original strain. The research suggests that applying heavy ion irradiation to protoplast is a possible method to breed mutant strains.

关键词

碳离子束辐照Clitopilus pinsitus原生质体高产变异株截短侧耳素

Keywords

Carbon ion beamIrradiationClitopilus pinsitus protoplastHigh-yielding mutant strainPleuromutilin

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