血清铁生物剂量计的可行性研究
Development of a new biodosimetry of serum iron
- 辐射研究与辐射工艺学报 2016年34卷第1期 页码：10501
- 作者机构：
1.南京航空航天大学 南京 210016
2.Nanjing University of Aeronautics & Astronautics,Nanjing 210016,China
- 作者简介：
谢丽华，女，1989年2月出生，2013年毕业于南华大学，现为南京航空航天大学在读硕士研究生，从事生物剂量计研究
张海黔，博士，教授，E-mail: zhanghq@nuaa.edu.cn
- 基金信息：国防基础科学研究项目(B2520133007)、国家自然科学基金(11575086, 31400721)、江苏省自然科学基金(BK20131355)和江苏高校优势学科建设工程项目资助 Supported by National Defense Basic Scientific Research Project (B252013307), National Nature Science Foundation of China(11575086, 31400721), Nature Science Foundation of Jiangsu Province (BK20131355) and Priority Academic Program Developmentof Jiangsu Higher Education Institutions
DOI：10.11889/j.1000-3436.2016.rrj.34.010501
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谢丽华, 张晓红, 胡晓丹, 等. 血清铁生物剂量计的可行性研究[J]. 辐射研究与辐射工艺学报, 2016,34(1):10501.
Lihua XIE, Xiaohong ZHANG, Xiaodan HU, et al. Development of a new biodosimetry of serum iron[J]. Journal of Radiation Research and Radiation Processing, 2016,34(1):10501.
谢丽华, 张晓红, 胡晓丹, 等. 血清铁生物剂量计的可行性研究[J]. 辐射研究与辐射工艺学报, 2016,34(1):10501. DOI： 10.11889/j.1000-3436.2016.rrj.34.010501.
Lihua XIE, Xiaohong ZHANG, Xiaodan HU, et al. Development of a new biodosimetry of serum iron[J]. Journal of Radiation Research and Radiation Processing, 2016,34(1):10501. DOI： 10.11889/j.1000-3436.2016.rrj.34.010501.
摘要
研究人体外周血血清经急性γ射线照射后血清铁的浓度变化，探讨其作为生物剂量计的可行性。人体血清经不同剂量γ射线辐照，采用酶联免疫吸附试验 (Enzymes linked immunosorbent assay,ELISA) 法定量检测血清铁的浓度，建立血清铁浓度与吸收剂量之间的剂量-效应关系。结果显示，血清铁浓度随吸收剂量升高，血清铁浓度与吸收剂量成线性平方关系：男性[,y,=30 676.5+2 383.5,x,-202.7,x,2, (,r,=0.99)]；女性 [,y,=26 974.4+ 1 611.8,x,-141.3,x,2, (,r,=0.98)]。4 Gy剂量时间-效应关系研究发现离体血清铁在2 d内维持稳定。利用建立的线性平方剂量-效应曲线估算的吸收剂量与双盲剂量(1、2、4 Gy)接近，估算值的95%置信区间较窄。研究结果提示血清铁具有重建人体吸收剂量的潜能。
Abstract
The aim is to study the serum iron changes ,in vitro, serum of human peripheral blood irradiated by Co-60 γ-rays and explore its feasibility as a biodosimetry. To establish the dose-response relationship,the concentration of serum iron in γ-rays irradiated serum was detected using ELISA (Enzymes linked immunosorbent assay). The results demonstrated that serum iron level increased with absorbed dose,and the dose-response curve of serum iron concentration followed the linear-quadratic model: male [,y,=30 676.5+2 383.5,x,-202.7,x,2, (,r,=0.99)],female [,y,=26 974.4+ 1 611.8,x,-141.3,x,2, (,r,=0.98)]. Time-response relationship of 4 Gy demonstrated that the serum iron was stable within 2 d. With the dose-response linear-quadratic curve,the absorbed dose was close to the double-blind dose (1,2 and 4 Gy),and the 95% confidence interval of the estimated value was narrow. Therefore,the serum iron has the potential to be a new biodosimetry to reconstruct human exposure dose.
关键词
生物剂量计血清铁γ射线
Keywords
Biological dosimeterSerum ironγ-rays
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