硫酸镁对人脐静脉血管内皮细胞的辐射防护作用
Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells
- 辐射研究与辐射工艺学报 2016年34卷第2期 页码：20201
- 作者机构：
1.苏州大学医学部放射医学与防护学院 苏州 215123
2.School of Radiation Medicine and Protection, Medical School of Soochow University, Suzhou 215123, China
- 作者简介：
孙阳,男,1989年7月出生,2012年毕业于苏州大学放医学院,现为该校硕士研究生,研究方向为放射损伤与防护
涂彧,博士,教授,E-mail:tuyu@suda.edu.com
- 基金信息：国家自然科学基金(K112830513)资助Supported by National Natural Science Foundation of China (K112830513)
DOI：10.11889/j.1000-3436.2016.rrj.34.020201
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孙阳, 涂彧. 硫酸镁对人脐静脉血管内皮细胞的辐射防护作用[J]. 辐射研究与辐射工艺学报, 2016,34(2):20201.
Yang SUN, Yu TU. Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells[J]. Journal of Radiation Research and Radiation Processing, 2016,34(2):20201.
孙阳, 涂彧. 硫酸镁对人脐静脉血管内皮细胞的辐射防护作用[J]. 辐射研究与辐射工艺学报, 2016,34(2):20201. DOI： 10.11889/j.1000-3436.2016.rrj.34.020201.
Yang SUN, Yu TU. Irradiation protection effects of magnesium sulfate on human umbilical vein endothelial cells[J]. Journal of Radiation Research and Radiation Processing, 2016,34(2):20201. DOI： 10.11889/j.1000-3436.2016.rrj.34.020201.
摘要
对数生长期的人脐静脉血管内皮细胞(Human umbilical vein endothelial cells, HUVECs) 分3组进行不同处理后采用流式细胞仪检测细胞周期分布;Annexin V/PI双染法测定细胞凋亡率;Western blot检测Bcl-2蛋白的表达情况;RT-PCR法检测Bcl-2 mRNA的表达情况。结果显示,硫酸镁可以在X-射线照射24 h后改善HUVECs细胞G2/M期阻滞;在辐照后24、48、72 h可以提高细胞对X-射线的辐射敏感性,并增加细胞凋亡率(,t,=4.24、7.19、3.20,p,<,0.05);在辐照后48、72 h能明显抑制抗凋亡蛋白Bcl-2蛋白的表达;照后24、48、72 h 的Bcl-2 mRNA表达量减少(,t,=3.034、6.182、3.957,p,<,0.05)。结果提示,硫酸镁可以缓解X-射线照射后细胞G2/M期阻滞,增加照射后HUVECs的细胞凋亡率,降低X-射线诱导的抗凋亡蛋白Bcl-2的表达。
Abstract
The human umbilical vein endothelial cells (HUVECs) of logarithmic growth phase were divided into three groups for different treatment. And then they were detected with the cell cycle distribution by flow cytometry, rate of apoptosis by annexin V/PI double staining method, expression of Bcl-2 protein by Western blot and expression of Bcl-2 mRNA by RT-PCR. The results showed that magnesium sulfate could advance the G2/M phase block of HUVECs after X-ray irradiation for 24 h, and improve the sensitivity of HUVECs to X-rays after irradiation for 24 h, 48 h and 72 h, increasing apoptosis rate to a certain extent (,t,=4.24, 7.19, 3.20,p,<,0.05) and decreasing Bcl-2 mRNA expression (,t,=3.034, 6.182, 3.957,p,<,0.05)after irradiation for 24 h, 48 h and 72 h, as well as inhibit the anti-apoptotic protein Bcl-2 protein expression significantly after irradiation for 48 h and 72 h. Therefore, magnesium sulfate could improve the G2/M phase arrest, increase the apoptosis rate and reduce Bcl-2 expression of HUVECs irradiated with X-rays.
关键词
硫酸镁人脐静脉血管内皮细胞X-射线细胞凋亡Bcl-2
Keywords
Magnesium sulfateHuman umbilical vein endothelial cellsX-raysCell apoptosisBcl-2
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