植物凝集素PHA-L对小鼠急性辐射防护作用
Protective effects of the plant lectin PHA-L on acute radiation injuries in mice
- 辐射研究与辐射工艺学报 2016年34卷第2期 页码：20203
- 作者机构：
1.中国医学科学院 北京协和医学院 放射医学研究所 天津 300192
2.Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China
- 作者简介：
张广慧,女,1987年5月出生,2011年毕业于泰山医学院,现为中国医学科学院放射医学研究所硕士研究生,研究方向为辐射防护食品药品研究,E-mail:iamzgh@yeah.net
龙伟,博士,副研究员,E-mail:longway@irm-cams.ac.cn
- 基金信息：国家自然科学基金青年项目(81202153)、教育部博士点新教师基金(20121106120042)、天津市应用基础与前沿技术研究计划青年项目(14JCQNJC10900)和中国医学科学院放射医学研究所发展基金(1523)资助Supported by National Natural Science Foundation of China(81202153), Doctoral Found of Ministry of Education(20121106120042),Tianjin application foundation and research in cutting-edge technologies program(14JCQNJC10900), and Development Fund ofInstitute of Radiation Medicine, Chinese Academy of Medical Sciences(1523)
DOI：10.11889/j.1000-3436.2016.rrj.34.020203
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张广慧, 吴红英, 靳瑾, 等. 植物凝集素PHA-L对小鼠急性辐射防护作用[J]. 辐射研究与辐射工艺学报, 2016,34(2):20203.
Guanghui ZHANG, Hongying WU, Jin JIN, et al. Protective effects of the plant lectin PHA-L on acute radiation injuries in mice[J]. Journal of Radiation Research and Radiation Processing, 2016,34(2):20203.
张广慧, 吴红英, 靳瑾, 等. 植物凝集素PHA-L对小鼠急性辐射防护作用[J]. 辐射研究与辐射工艺学报, 2016,34(2):20203. DOI： 10.11889/j.1000-3436.2016.rrj.34.020203.
Guanghui ZHANG, Hongying WU, Jin JIN, et al. Protective effects of the plant lectin PHA-L on acute radiation injuries in mice[J]. Journal of Radiation Research and Radiation Processing, 2016,34(2):20203. DOI： 10.11889/j.1000-3436.2016.rrj.34.020203.
摘要
观察植物凝集素PHA-L对小鼠急性辐射损伤的保护作用及相关机制。采用动物30 d存活率实验,即小鼠全身辐照至吸收剂量为7.2 Gy后观察其存活情况;利用外周血和免疫学实验,小鼠全身照射至吸收剂量为7.2 Gy后测外周血白细胞数(White blood cell, WBC)、股骨有核细胞数(The number of nucleated cells in bone marrow, BMNC)、骨髓DNA含量以及各脏器指数;对小鼠进行9.0 Gy腹部照射,取小肠组织做病理切片观察。结果显示,照射给药组小鼠30 d存活率比单纯照射组均有提高,特别是高剂量组提高约60%;与单纯照射组相比,照射给药高剂量组的白细胞数、骨髓DNA含量和脾结节数均有提高,分别从0.74±0.16、1.02±0.17和9.80±6.46提高到1.18±0.40、1.22±0.17和18.10±6.87,数据均有统计学意义(,p,<,0.05),并且PHA-L对脏器也有一定的保护作用;与单纯照射组相比,照射给药高剂量组小鼠肠道组织损伤恢复明显。提示植物凝集素PHA-L对急性辐射损伤具有保护作用,但其作用机制有待进一步研究。
Abstract
The aim is to observe the protective effects of plant lectin PHA-L against radiation injuries and the relative mechanism. 30 d survival experiment was used to observe the survival condition of mice 30 d after irradiation with 7.2 Gy γ-rays. Blood and immunological experiments of mice were carried out to observe WBC (White blood cell), femoral nucleated cells, bone marrow DNA content and organs index after irradiation with 7.2 Gy γ-rays. Intestine pathological section of mice irradiated on abdomen with 9.0 Gy γ-rays was prepared to observe the pathology of intestine. The results showed that compared with the irradiation group, the survival rate of mice treated with PHA-L increased, especially for the high dose group (60% lifted); compared with the irradiation group, white blood cell count, bone marrow DNA content and spleen nodules of mice administered with high dose group increased from 0.74±0.16, 1.02±0.17 and 9.80±6.46 to 1.18±0.40, 1.22±0.17 and 18.10±6.87 respectively, which were statistically significant (,p,<,0.05). Moreover, PHA-L also had some protective effect on the organ. In pathology observation, the intestines of mice treated with PHA-L recovered significantly as well. Therefore, plant lectin PHA-L can protect mice from radiation injuries, yet the mechanism needs further study.
关键词
辐射损伤植物凝集素PHA-L放射性肠炎
Keywords
Radiation damagePlant lectin PHA-LRadiation intestine
references
Coleman C N , Blakely W F , Fike J R , 等 . Molecular and cellular biology of moderate-dose (1~10 Gy) radiation and potential mechanisms of radiation protection: report of a workshop at Bethesda, Maryland, December 17-18, 2001 . Radiation Research , 2003 . 159 (6 ):812 -834. .
Meng A , Wang Y , Brown S A , 等 . Ionizing radiation and busulfan inhibit murine bone marrow cell hematopoietic function via apoptosis-dependent and-independent mechanisms . Experimental Hematology , 2003 . 31 (12 ):1348 -1356. .
Meng A , Wang Y , Van Zant G , 等 . Ionizing radiation and busulfan induce premature senescence in murine bone marrow hematopoietic cells . Cancer Research , 2003 . 63 (17 ):5414 -5419. .
侯 朝华 , 霍 彬 , 宋 杨 . 组织间近距离放射性粒子植入治疗肿瘤的进展 . 国际放射医学核医学杂志 , 2015 . 39 (4 ):348 -351. .
HOU Zhaohua , HUO Bin , SONG Yang . Organizational close radioactive seed implantation therapy in treating tumor progression . Radiation Medicine and Unclear Medicine , 2015 . 39 (4 ):348 -351. .
Acharya S , Sanjeev G , Bhat N N , 等 . The effect of electron and gamma irradiation on the induction of micronuclei in cytokinesis-blocked human blood lymphocytes . Radiation and Environmental Biophysics , 2009 . 48 (2 ):197 -203. .
Cook T , Wang Z , Alber S , 等 . Nitric oxide and ionizing radiation synergistically promote apoptosis and growth inhibition of cancer by activating p53 . Cancer Research , 2004 . 64 (21 ):8015 -8021. .
Landsverk K S , Lyng H , Stokke T . The response of malignant B lymphocytes to ionizing radiation: cell cycle arrest, apoptosis and protection against the cytotoxic effects of the mitotic inhibitor nocodazole . Radiation Research , 2004 . 162 (4 ):405 -415. .
Yamauchi R , Morita A , Yasuda Y , 等 . Different susceptibility of malignant versus nonmalignant human T cells toward ultraviolet A-1 radiation-induced apoptosis . Journal of Investigative Dermatology , 2004 . 122 (2 ):477 -483. .
张 春生 , 彭 珊茁 . 抗氧化物质在辐射防护中的作用 . 中国工业医学杂志 , 2003 . 16 (4 ):222 -224. .
ZHANG Chunsheng , PENG Shanzhuo . Effects of antioxidant substances on radioprotection . Chinese Journal of Industrial Medicine , 2003 . 16 (4 ):222 -224. .
Chaudière J , Ferrari-Iliou R . Intracellular antioxidants: from chemical to biochemical mechanisms . Food and Chemical Toxicology , 1999 . 37 (9 ):949 -962. .
鲍 锦库 . 植物凝集素的功能 . 生命科学 , 2011 . 23 533 -540. .
BAO Jinku . Plant lectins: multifunctions and applications . Chinese Bulletin of Life Sciences , 2011 . 23 533 -540. .
王 鼎 , 宋 兵 , 钟 旋 , 等 . 植物凝集素刺激外周血单个核细胞增殖及分泌因子表达的变化 . 中国组织工程研究 , 2014 . 18 (23 ):3707 -3714. .
WANG Ding , SONG Bing , ZHONG Xuan , 等 . Phytohaemagglutinin stimulates the proliferation of peripheral blood mononuclear cells and expression of secretory cytokines . Chinese Journal of Tissue Engineering Research , 2014 . 18 (23 ):3707 -3714. .
Unitt J , Hornigold D . Plant lectins are novel Toll-like receptor agonists . Biochem Pharmacol , 2011 . 81 1324 -1328. .
Xu Y I , Jagannath C , Liu X D , 等 . Toll-like receptor 4 is a sensor for autophagy associated with innate immunity . Immunity , 2007 . 27 (1 ):135 -144. .
路 璐 , 张 俊伶 , 李 德冠 , 等 . 6 Gy 137Cs g-射线照射对小鼠造血功能损伤的动态观察研究 . 国际放射医学核医学杂志 , 2015 . 39 (5 ):393 -396. .
LU Lu , ZHANG Junling , LI Deguan , 等 . Effects of 6 Gy 137Cs g-irradiattion on the hematopoietic system of mice . International Medicine and Nuclear Medicine , 2015 . 39 (5 ):393 -396. .
张 俊伶 , 路 璐 , 李 德冠 , 等 . 基因芯片表达分析 4 Gy g射线对小鼠骨髓c-kit 阳性细胞影响 . 国际放射医学核医学杂志 , 2015 . 39 (2 ):116 -120. .
ZHANG Junling , LU Lu , LI Deguan , 等 . Expression changes of genes in c-kit positive cells after 4 Gy g-ray irradiation: a gene chip analysis . International Medicine and Nuclear Medicine , 2015 . 39 (2 ):116 -120. .
Deretic V . Multiple regulatory and effector roles of autophagy in immunity . Current Opinion in Immunology , 2009 . 21 (1 ):53 -62. .
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