耐辐射奇球菌
DR_2327 和DR_2328 基因克隆及功能分析Cloning, and functional analysis of
DR_2327 andDR_2328 in Deinococcus radiodurans- 辐射研究与辐射工艺学报 2016年34卷第5期
- 作者机构：
1.永州职业技术学院 永州 425100
- 作者简介：
[ "杨宇虹, 女, 1984年10月出生, 2015年于南华大学获理学硕士学位, 讲师, E-mail:yyh_100@163.com", "YANG Yuhong (female) was born in October 1984 and obtained a master degree from University of South China in 2015, Lecturer, E-mail: yyh_100@163.com" ]
- 基金信息：
DOI：10.11889/j.1000-3436.2016.rrj.34.050203
中图分类号：
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杨宇虹. 耐辐射奇球菌
DR_2327 和DR_2328 基因克隆及功能分析[J]. 辐射研究与辐射工艺学报, 2016,34(5):050203-8.Yuhong YANG. Cloning, and functional analysis of
DR_2327 andDR_2328 in Deinococcus radiodurans[J]. Journal of Radiation Research and Radiation Processing, 2016,34(5):050203-8.杨宇虹. 耐辐射奇球菌
DR_2327 和DR_2328 基因克隆及功能分析[J]. 辐射研究与辐射工艺学报, 2016,34(5):050203-8. DOI： 10.11889/j.1000-3436.2016.rrj.34.050203.Yuhong YANG. Cloning, and functional analysis of
DR_2327 andDR_2328 in Deinococcus radiodurans[J]. Journal of Radiation Research and Radiation Processing, 2016,34(5):050203-8. DOI： 10.11889/j.1000-3436.2016.rrj.34.050203.
摘要
通过生物信息学分析耐辐射奇球菌,DR_2327,和,DR_2328,基因及编码蛋白的基本性质，利用PCR方法克隆,DR_2327,和,DR_2328,的全基因，连接至pGEM-T载体上，转化至大肠杆菌JM109中，并进行双酶切鉴定。生物信息学分析结果表明，,DR_2327,和,DR_2328,基因位于同一操纵子中，且DR_2328蛋白具有组氨酸激酶活性，DR_2327蛋白具有反应调节子功能，提示两者很可能组成一对双组分系统。体外实验表明，,DR_2327,和,DR_2328,基因的外源表达在一定程度上提高了大肠杆菌对紫外辐照和H,2,O,2,的耐受能力。
Abstract
The main characteristics of ,DR_2327,and ,DR_2328, in Deinococcus radiodurans were analyzeed by bioinformatics. Full-length gene of ,DR_2327, and ,DR_2328, was amplified by PCR, and constructed in pGEM-T vector. Transformed recombinant vector into E.coli JM109 and followed by restriction enzyme digestion. ,DR_2327, and ,DR_2328, gene was cloned successfully. Bioinformatic analysis results showed that ,DR_2327, and ,DR_2328, gene located in the same operon, and DR_2328 protein with histidine kinase activity, DR_2327 protein with response regulator activity. It is suggested that ,DR_2327, and ,DR_2328, constitute a two-component system in Deinococcus radiodurans. ,In vitro, experiments showed that ,DR_2327, and ,DR_2328, could enhance tolerance to UV radiation and H,2,O,2, of ,E.coli,.
关键词
耐辐射奇球菌DR_2327DR_2328基因克隆生物信息学分析
Keywords
Deinococcus radioduransDR_2327DR_2328Gene cloningBioinformatics analysis
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